CTCF (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC, A

Length: 2184 Sequence: atggaaggtg atgcagtcga agccattgtg gaggagtccg aaacttttat taaaggaaag gagagaaaga cttaccagag acgccgggaa gggggccagg aagaagatgc ctgccactta ccccagaacc agacggatgg gggtgaggtg gtccaggatg tcaacagcag tgtacagatg gtgatgatgg aacagctgga ccccaccctt cttcagatga agactgaagt aatggagggc acagtggctc cagaagcaga ggctgctgtg gacgataccc agattataac tttacaggtt gtaaatatgg aggaacagcc cataaacata ggagaacttc agcttgttca agtacctgtt cctgtgactg tacctgttgc taccacttca gtagaagaac ttcagggggc ttatgaaaat gaagtgtcta aagagggcct tgcggaaagt gaacccatga tatgccacac cctacctttg cctgaagggt ttcaggtggt taaagtgggg gccaatggag aggtggagac actagaacaa ggggaacttc caccccagga agatcctagt tggcaaaaag acccagacta tcagccacca gccaaaaaaa caaagaaaac caaaaagagc aaactgcgtt atacagagga gggcaaagat gtagatgtgt ctgtctacga ttttgaggaa gaacagcagg agggtctgct atcagaggtt aatgcagaga aagtggttgg taatatgaag cctccaaagc caacaaaaat taaaaagaaa ggtgtaaaga agacattcca gtgtgagctt tgcagttaca cgtgtccacg gcgttcaaat ttggatcgtc acatgaaaag ccacactgat gagagaccac acaagtgcca tctctgtggc agggcattca gaacagtcac cctcctgagg aatcacctta acacacacac aggtactcgt cctcacaagt gcccagactg cgacatggcc tttgtgacca gtggagaatt ggttcggcat cgtcgttaca aacacaccca cgagaagcca ttcaagtgtt ccatgtgcga ttacgccagt gtagaagtca gcaaattaaa acgtcacatt cgctctcata ctggagagcg tccgtttcag tgcagtttgt gcagttatgc cagcagggac acatacaagc tgaaaaggca catgagaacc cattcagggg aaaagcctta tgaatgttat atttgtcatg ctcggtttac ccaaagtggt accatgaaga tgcacatttt acagaagcac acagaaaatg tggccaaatt tcactgtccc cactgtgaca cagtcatagc ccgaaaaagt gatttgggtg tccacttgcg aaagcagcat tcctatattg agcaaggcaa gaaatgccgt tactgtgatg ctgtgtttca tgagcgctat gccctcatcc agcatcagaa gtcacacaag aatgagaagc gctttaagtg tgaccagtgt gattacgctt gtagacagga gaggcacatg atcatgcaca agcgcaccca caccggggag aagccttacg cctgcagcca ctgcgataag accttccgcc agaagcagct tctcgacatg cacttcaagc gctatcacga ccccaacttc gtccctgcgg cttttgtctg ttctaagtgt gggaaaacat ttacacgtcg gaataccatg gcaagacatg ctgataattg tgctggccca gatggcgtag agggggaaaa tggaggagaa acgaagaaga gtaaacgtgg aagaaaaaga aagatgcgct ctaagaaaga agattcctct gacagtgaaa atgctgaacc agatctggac gacaatgagg atgaggagga gcctgccgta gaaattgaac ctgagccaga gcctcagcct gtgaccccag ccccaccacc cgccaagaag cggagaggac gaccccctgg cagaaccaac cagcccaaac agaaccagcc aacagctatc attcaggttg aagaccagaa tacaggtgca attgagaaca ttatagttga agtaaaaaaa gagccagatg ctgagcccgc agagggagag gaagaggagg cccagccagc tgccacagat gcccccaacg gagacctcac gcccgagatg atcctcagca tgatggaccg Protein function: Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays an important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. Plays a role in the recruitment of CENPE to the pericentromeric/centromeric regions of the chromosome during mitosis (PubMed:26321640). [The UniProt Consortium]