COX4NB (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 633 Sequence: atgcccgggg tgaaactgac cacccaggcc tactgcaaga tggtgctgca cggcgccaag tacccgcact gcgccgtcaa cgggctcctg gtggccgaga agcagaagcc gcgtaaggag cacctccccc tgggcggccc cggcgcccac cacaccctct tcgtggactg catccccctc ttccacggca ccctggccct cgcccccatg ctggaggtgg ctctcaccct gattgattca tggtgcaaag atcatagcta cgtgattgct ggttattatc aagctaatga gcgagtaaag gatgccagtc caaaccaggt tgcagagaag gtggcctcca gaatcgccga gggcttcagc gacactgcgc tcatcatggt agacaacacc aagtttacga tggactgcgt agcgcctacg atccacgtgt acgagcacca tgagaacaga tggcggtgca gagacccaca ccatgactac tgtgaagact ggccagaggc acagaggatc tcagcctcgc tcctggacag ccggtcctac gagacgctcg tggatttcga taaccacctg gatgacattc ggaatgactg gacaaaccca gagatcaata aagctgtcct acacttgtgc Protein function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176, PubMed:32439656). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N- terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable). [The UniProt Consortium]