AP2A2 (Vector pUC, Accession No. BC006155)

Length: 2820 Sequence: atgccggccg tgtccaaggg ggacgggatg cggggcctgg cggtcttcat ctcggatatc cgcaactgta aaagtaaaga agcagaaata aaaaggataa acaaggaact ggcaaatatc agatcaaaat ttaaaggtga caaggctctt gatggctata gtaaaaaaaa gtacgtctgc aagttgctct tcatctttct ccttggtcat gacattgact ttggacacat ggaggctgtg aacctgctga gttcaaacag atacacggaa aagcagatcg gctacctttt catctctgtg ttggtgaact caaacagtga gctgatccgc ctgatcaaca acgccatcaa gaatgacctg gccagccgca accccacctt catgggcctg gccctgcact gcatcgccag cgtgggcagc cgggagatgg ccgaggcctt cgccggggag atccctaagg tcctcgtagc cggagacact atggacagcg tgaagcagag cgcggccctg tgcttgctgc gcctgtacag gacgtccccc gatcttgtcc ccatgggcga ctggacatcc cgagtggtgc acctgctcaa tgaccagcac ttgggtgtgg taactgcagc cacaagtctg atcaccactt tagcacagaa gaacccagaa gagtttaaaa cctccgtgtc tctggctgtc tctaggctaa gcagaatcgt gacgtctgca tccacagatc tccaggatta cacttactat tttgtcccgg ctccctggct gtctgtcaaa ctgctgagac tgctgcagtg ctacccaccc ccagaccctg cagtgcgagg ccgcctgact gagtgcctgg agaccatcct gaacaaagcc caagaaccgc ccaagtcgaa gaaggtccag cactccaacg cgaagaatgc cgtgctcttc gaggccatca gcttaatcat tcaccatgac agtgagccga acctgctcgt ccgtgcctgc aaccagttgg gccagtttct gcagcaccgc gagaccaacc tgcgctacct ggccctggag agcatgtgca cgctggccag ctctgagttc tcccatgagg ctgtcaagac gcacatcgag acggtcatca acgccctgaa gactgagcgg gacgtgagcg tgcggcagcg ggccgtggac ctcctctacg ccatgtgcga ccgcagcaac gccccacaga tcgtggccga gatgctgagc tatctggaga cagctgacta ctccatccga gaagagattg tgctgaaggt cgccatcctg gctgagaagt acgcggtgga ctacacctgg tatgtggata ccatcttgaa cttgatccga attgctggtg attacgtgag tgaagaggtg tggtaccgag tcattcagat cgtcatcaac cgggacgacg tgcagggcta cgcggccaag actgtgttcg aggctcttca ggctcccgcg tgccacgaga acctggtcaa agtgggcggc tacatcctgg gggagtttgg aaacttgata gctggagacc cgagatccag cccgctgatc cagttccacc tgctgcactc caagttccac ctgtgcagcg tccccacccg cgcgctgctc ctgtccacct acatcaagtt cgtgaacctc ttcccggagg tgaagcccac catccaggac gtgctgcgca gcgacagcca gctcaggaac gcagacgtgg agctgcagca gcgtgctgtg gagtacctgc ggctcagcac cgtggccagc accgacattc tggcgaccgt gctggaggag atgcccccat tcccggagcg ggagtcctcc atcttggcaa agctcaagaa gaagaagggc cccagcacgg tgacagacct ggaggacacc aagcgggaca ggagtgtgga cgtgaacggg ggtcctgagc ctgccccagc cagtaccagc gccgtgtcta cgccttctcc gtcggcagac ctgctgggtc tcggggctgc cccccctgcc cccgcgggcc ccccaccctc ctccggcggc agcgggctgc tcgtggacgt gttctcagac tcggcctctg tggtcgcgcc tctcgctcct ggctccgaag acaactttgc caggtttgtt tgtaaaaaca atggtgtgtt gtttgaaaac cagctgcttc aaattggact taagtctgaa tttcggcaga atttaggtcg gatgtttatc ttttatggta ataagacctc cacgcagttc ctaaacttta ccccaacact aatctgttca gacgaccttc agcctaacct gaacctgcag accaagcccg tggacccgac cgtggagggg ggcgcgcagg tgcagcaggt ggtcaacata gagtgcgtgt ccgacttcac ggaggcgcca gtcctcaaca ttcagttcag gtatgggggc accttccaga acgtgtctgt gcagctgccc atcactctca acaaattctt ccagccgaca gaaatggctt ctcaggattt ctttcaacgt tggaagcagt tgagcaatcc acagcaggaa gtgcagaaca tcttcaaagc aaagcaccca atggacacag aagtcaccaa agccaagatc attggatttg gttctgcact tcttgaagaa gttgatccta atcctgcgaa tttcgtggga gctggaatca tccacacgaa aaccacccag attggatgcc tgctgcgctt ggagccgaac ctgcaagccc agatgtaccg gctcacgctg cgcacaagta aggaagccgt ttctcagaga ttatgtgaat tgctctcagc Protein function: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L- [LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non- clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed:23676497). The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. [The UniProt Consortium]