ALOXE3 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 2136 Sequence: ATGGCAGTGT ACCGCCTGTG TGTGACCACT GGTCCCTACC TGAGGGCCGG CACACTGGAC AACATCTCTG TCACACTGGT GGGCACGTGT GGTGAAAGCC CCAAGCAGCG GCTAGATCGA ATGGGCAGGG ACTTCGCCCC TGGATCGGTA CAGAAGTACA AGGTGCGTTG CACAGCGGAG CTGGGTGAGC TCTTGCTGCT GCGTGTACAC AAGGAGCGCT ACGCTTTCTT CCGCAAGGAC TCTTGGTACT GTAGCCGCAT CTGTGTCACC GAACCGGATG GTAGTGTATC CCACTTCCCC TGCTATCAGT GGATTGAAGG CTACTGCACC GTGGAGCTGA GGCCAGGAAC AGCAAGAACT ATTTGTCAGG ACTCTCTTCC CCTCCTCCTG GATCACAGGA CACGGGAGCT CCGGGCCCGA CAAGAATGCT ACCGCTGGAA GATCTATGCC CCTGGCTTCC CCTGCATGGT AGACGTCAAC AGCTTTCAGG AGATGGAGTC AGACAAGAAA TTTGCCTTGA CAAAGACGAC AACTTGTGTA GACCAGGGTG ACAGCAGTGG GAATCGGTAC CTGCCCGGCT TCCCCATGAA AATTGACATC CCATCCCTGA TGTACATGGA GCCCAATGTT CGATACTCAG CCACCAAGAC GATCTCGCTG CTCTTCAATG CCATCCCTGC GTCCTTGGGA ATGAAGCTTC GAGGGCTGTT GGATCGCAAG GGCTCCTGGA AGAAGCTGGA TGACATGCAG AACATCTTCT GGTGCCATAA GACCTTCACG ACAAAGTATG TCACAGAGCA CTGGTGTGAA GATCACTTCT TTGGGTACCA GTACCTGAAT GGTGTCAATC CCGTCATGCT CCACTGCATC TCTAGCTTGC CCAGCAAGCT GCCTGTCACC AATGACATGG TGGCCCCCTT GCTGGGACAG GACACATGCC TGCAGACAGA GCTAGAGAGG GGGAACATCT TCCTAGCGGA CTACTGGATC CTGGCGGAGG CCCCCACCCA CTGCCTAAAC GGCCGCCAGC AGTACGTGGC CGCCCCACTG TGCCTGCTGT GGCTCAGCCC CCAGGGGGCG CTGGTGCCCT TGGCCATCCA GCTCAGCCAG ACCCCCGGGC CTGACAGCCC CATCTTCCTG CCCACTGACT CCGAATGGGA CTGGCTGCTG GCCAAGACGT GGGTGCGCAA CTCTGAGTTC CTGGTGCACG AAAACAACAC GCACTTTCTG TGCACGCATT TGCTGTGCGA GGCCTTCGCC ATGGCCACGC TGCGCCAGCT GCCGCTCTGC CACCCCATCT ACAAGCTCCT ACTCCCCCAC ACTCGATACA CGCTGCAGGT GAACACCATC GCGAGGGCCA CGCTGCTCAA CCCCGAGGGC CTCGTGGACC AGGTCACGTC CATCGGGAGG CAAGGCCTCA TCTACCTCAT GAGCACGGGC CTGGCCCACT TCACCTACAC CAATTTCTGC CTTCCGGACA GCCTGCGGGC CCGCGGCGTC CTGGCTATCC CCAACTACCA CTACCGAGAC GACGGCCTGA AGATCTGGGC GGCCATTGAG AGCTTTGTCT CAGAAATCGT GGGCTACTAT TATCCCAGTG ACGCATCTGT GCAGCAGGAT TCGGAGCTGC AGGCCTGGAC TGGCGAGATT TTTGCTCAGG CGTTCCTGGG CCGGGAAAGC TCAGGTTTCC CAAGCCGGCT GTGCACCCCA GGAGAGATGG TGAAGTTCCT CACTGCAATC ATCTTCAATT GCTCTGCCCA GCACGCTGCT GTCAACAGTG GGCAGCATGA CTTTGGGGCC TGGATGCCCA ATGCTCCATC ATCCATGAGG CAGCCCCCAC CCCAGACCAA GGGGACCACC ACCCTGAAGA CTTACCTAGA CACCCTCCCT GAAGTGAACA TCAGCTGTAA CAACCTCCTC CTCTTCTGGT TGGTTAGCCA AGAACCCAAG GACCAGAGGC CCCTGGGCAC CTACCCAGAT GAGCACTTCA CAGAGGAGGC CCCGAGGCGG AGCATCGCCG CCTTCCAGAG CCGCCTGGCC CAGATCTCAA GGGACATCCA GGAGCGGAAC CAGGGTCTGG CACTGCCCTA CACCTACCTG GACCCTCCCC TCATTGAGAA CAGCGTCTCC Protein function: Non-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced lipoxygenases activity (PubMed:12881489, PubMed:17045234, PubMed:20921226, PubMed:20923767). The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols and ketones (PubMed:12881489, PubMed:17045234, PubMed:20923767). In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides (PubMed:20921226). In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins (PubMed:21558561). Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss (PubMed:21558561). In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites (PubMed:21558561). Plays also a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activate PPARG. Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia. [The UniProt Consortium]