ALKBH8 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 717 Sequence: atggacagca accatcaaag taattacaaa ctcagtaaaa ctgagaagaa gttcttaagg aaacagatta aagccaagca tactttgctg agacatgaag gcattgagac agtatcctat gccactcaga gcctggttgt tgccaatggt ggtttgggta atggtgtgag tcggaaccag ctgctcccgg ttttagagaa atgtggactg gtggatgctc tcttaatgcc acctaacaag ccgtactcat ttgcaagata cagaactaca gaagaatcta agagagccta tgttaccctc aatggaaaag aagtagtgga tgatttagga caaaagatca ctctgtattt gaattttgtg gaaaaagtgc agtggaagga gttgaggcct caagccttac caccaggact catggtagta gaagaaataa tttcttctga ggaggagaaa atgcttttgg aaagtgttga ttggacagaa gatacagaca atcaaaactc tcaaaaatcc ttaaaacaca gaagagtaaa gcattttggt tatgagttcc actatgagaa caacaatgta gataaagata agccattatc tgggggtctt cctgacattt gtgaaagctt tttggagaaa tggttgagga aaggttacat taaacataaa cctgatcaaa tgaccataaa tcagtatgaa cctgggcaag attgtcatgg Protein function: Catalyzes the methylation of 5-carboxymethyl uridine to 5- methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323, PubMed:31079898). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)- methoxycarbonylhydroxymethyluridine, has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182). [The UniProt Consortium]