25-hydroxy Cholesterol-d2

25-hydroxy Cholesterol-d2 is intended for use as an internal standard for the quantification of 25-hydroxy cholesterol (Cay-11097) by GC- or LC-MS. 25-hydroxy Cholesterol is an oxysterol. It is formed from cholesterol by cholesterol-25-hydroxylase, and its production can be induced by inflammation or infection. 25-hydroxy Cholesterol suppresses endogenous cholesterol synthesis by binding to insulin-induced gene (INSIG) proteins and preventing sterol regulatory element binding proteins (SREBPs) from being transported to the Golgi. It inhibits IgA class switching induced by LPS and various cytokines in B cells (IC50 = ~50 nM). 25-hydroxy Cholesterol inhibits replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Vero cells (EC50 = 3. µM) and reduces increases in viral protein production in infected Vero cells when used prior to infection. It reduces viral RNA loads in the lung and trachea in a mouse model of SARS-CoV-2 infection when administered at a dose of 100 mg/kg per day. Serum levels of 25-hydroxy cholesterol are increased in patients with SARS-CoV-2. Formal Name: (3S,8S,9S,10R,13R,14S,17R)-17-((R)-6-hydroxy-6-methylheptan-2-yl)-10,13-dimethyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-7,7-d2-3-ol. Molecular Formula: C27H44D2O2. Formula Weight: 404.7. Purity: >99% deuterated forms (d1-d2). Formulation (Request formulation change): A solid. Solubility: Chloroform: Soluble: >10 mg/ml Methanol: Sparingly soluble: 1-10 mg/ml. SMILES: [H][C@]12[C@]([H])(CC[C@]3([C@]2(CC[C@]3([H])[C@@H](CCCC(C)(O)C)C)[H])C)[C@@]4(C)CC[C@@H](CC4=CC1([2H])[2H])O. InChi Code: InChI=1S/C27H46O2/c1-18(7-6-14-25(2,3)29)22-10-11-23-21-9-8-19-17-20(28)12-15-26(19,4)24(21)13-16-27(22,23)5/h8,18,20-24,28-29H,6-7,9-17H2,1-5H3/t18-,20+,21+,22-,23+,24+,26+,27-/m1/s1/i9D2. InChi Key: INBGSXNNRGWLJU-HACJLSAASA-N. Storage: -20°C. Stability: > 4 years.