THP1 Lipid

THP1 lipid is an ionizable cationic lipid (pKa = 5.451) that has been used in the generation of lipid nanoparticles (LNPs) for the delivery of mRNA in vitro and in vivo. LNPs containing THP1 lipid and encapsulating mRNA encoding luciferase induce luciferase expression in HEK293 and RAW 264. cells. LNPs containing THP1 lipid and 1-palmitoyl-2-oleoyl-sn-glycero-3-PE (1,2-POPE, Cay-20067) accumulate in mouse liver after intravenous administration and have a higher transfection efficiency than LNPs containing DLin-MC3-DMA (Cay-34364). THP1 lipid-containing LNPs encapsulating Cre mRNA induce the expression of tdTomato in the liver of Ai14 mice engineered to express tdTomato upon Cre-mediated recombination.. Solulibility: Ethanol: Soluble: =10 mg/mL: .