O12-D3-I3

An ionizable cationic lipid (pKa = 6.39), has been used in the generation of LNPs for siRNA delivery in vitro and in vivo, LNPs containing O12-D3-I3 and encapsulating ferrocene and siRNA targeting Nrf2 or system xc- cystine/glutamate transporter decrease viability of HepG2 cells, LNPs containing O12-D3-I3 and an mRNA reporter selectively accumulate in the tumor and liver over the lungs, kidneys, spleen, and heart in mice, LNPs containing O12-D3-I3 and encapsulating ferrocene and siRNA targeting Nrf2 or system xc- cystine/glutamate transporter reduce tumor growth and volume in an MCF-7 breast cancer mouse xenograft model, LNPs containing O12-D3-I3 and encapsulating siRNA targeting Factor VII decrease hepatocyte Factor VII mRNA levels in mice. Formulation: A solution in ethanol. InChI: InChI=1S/C39H73N5O5/c1-3-5-7-9-11-13-15-17-19-21-30-48-37(45)24-28-44(27-23-26-42-39(47)36(40)32-35-33-41-34-43-35)29-25-38(46)49-31-22-20-18-16-14-12-10-8-6-4-2/h33-34,36H,3-32,40H2,1-2H3,(H,41,43)(H,42,47). InChIKey: WRGZOVIIXQZLPE-UHFFFAOYSA-N. SMILES: O=C(NCCCN(CCC(OCCCCCCCCCCCC)=O)CCC(OCCCCCCCCCCCC)=O)C(N)CC1=CNC=N1