Ionizable Lipid 4

Ionizable lipid 4 is an ionizable cationic lipid (pKa = 6.1) and a hydrogen peroxide-induced rearrangement product of the cationic lipid CA-lipid 5. Charge-altering lipid nanoparticles (CALNPs) containing CA-lipid 5 and encapsulating siRNA against EGFP undergo hydrogen peroxide-induced removal of the phenylboronic acid groups from CA-lipid 5 in MCF-7 cells in vitro, generating ionizable lipid 4-containing LNPs with reduced positive charges at physiological pH, facilitating intracellular siRNA release and gene silencing. LNPs containing ionizable lipid 4 and encapsulating siRNA against PLK1 reduce tumor volume in an MCF-7 mouse xenograft model less effectively than CALNPs containing CA-lipid 5.Formal Name: 9,12-octadecadienoic acid, (9Z,12Z)-1,1'-[2-[[[[3-(diethylamino)propoxy]carbonyl]oxy]methyl]-1,3-propanediyl] ester. CAS Number: 1799316-81-6. Molecular Formula: C48H85NO7. Formula Weight: 788.2. Purity: >95%. Formulation: (Request formulation change), A solution in methyl acetate. Solubility: Ethanol: Sparingly soluble: 1-10 mg/ml, PBS (pH 7.2): Slightly soluble: 0.1-1 mg/ml. SMILES: CCCCC/C=C\C/C=C\CCCCCCCC(OCC(COC(CCCCCCC/C=C\C/C=C\CCCCC)=O)COC(OCCCN(CC)CC)=O)=O. InChi Code: InChI=1S/C48H85NO7/c1-5-9-11-13-15-17-19-21-23-25-27-29-31-33-35-38-46(50)54-42-45(44-56-48(52)53-41-37-40-49(7-3)8-4)43-55-47(51)39-36-34-32-30-28-26-24-22-20-18-16-14-12-10-6-2/h15-18,21-24,45H,5-14,19-20,25-44H2,1-4H3/b17-15-,18-16-,23-21-,24-22-. InChi Key: CKINQEPXJRAYIN-MLLZQYMOSA-N.