COX-2 (ovine) Electrophoresis Standard

Cyclooxygenase 2 (COX-2) is a bifunctional enzyme that exhibits both COX and peroxidase activities and catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins. The COX component converts arachidonic acid to the hydroperoxy endoperoxide prostaglandin G2 (PGG2, Cay-17010), and the peroxidase component reduces the endoperoxide to the corresponding alcohol PGH2 (Cay-17020). COX2 expression is induced by a variety of stimuli, including phorbol esters, LPS, and cytokines and is responsible for the biosynthesis of PGs under acute inflammatory conditions. Thus, COX-2 has been the focus of attention for nonsteroidal anti-inflammatory drug (NSAID) development. This product is intended to be used as a standard for Western blot (WB) and electrophoresis. This enzyme has been carefully purified to exclude all unrelated proteins and may not be catalytically active.Synonyms: Cyclooxygenase 2, PGHS-2, Prostaglandin H Synthase 2. Purity: >95%. Source: Isolated from sheep placenta. MW: 72 kDa/subunit. Formulation: (Request formulation change), 5 µg of purified COX-2. Storage Buffer: Laemmli buffer (2% SDS, 10% glycerol, 0.70 M Tris-HCl, pH 6.8, 1.5% 2-mercaptoethanol, 0.04 % bromophenol blue). Applications: Western blot and electrophoresis.