xtraFluor(TM) Violet 420 Maleimide

xtraFluor(TM) Violet 420 (XV420) Maleimide is a thiol-reactive XV420 dye that can be readily used for labeling antibodies and other thiol-containing molecules such as peptides and thiol-modified oligos. XV420 is an extremely bright violet laser-excitable fluorophore. With a maximum excitation well matched the 405 nm violet laser and an emission peak at ~420 nm, XV420 can be conveniently used to replace BD Horizon Brilliant(TM) Violet 421 (BV421). It can be used on flow cytometers equipped with a violet laser and appropriate filters for large multicolor flow cytometry panels. It is a brighter alternative to Pacific Blue (ThermoFisher) and BD Horizon(TM) V450 (BD Biosciences). XV420 enables improved resolution for dim population identification. It is particularly useful in multicolor applications where it can be used to better resolve dim populations. XV420 dye offers new options for multicolor panel design through the use of a violet laser. Additionally, the dye's spillover properties, stability under light and in standard buffers, and compatibility with blood collection tubes offer ease-of-use for a range of applications.