XFD790 PEG4 DBCO

XFD790, manufactured by AAT Bioquest, is a highly efficient near-infrared fluorescent dye that is structurally similar to Alexa Fluor(TM) 790 (Thermo Fisher). Spectrally analogous to indocyanine green (ICG) and IRDye(TM) 800, XFD790 demonstrates exceptional aqueous solubility and sustained fluorescence stability over a broad pH range (pH 4-10), ensuring consistent and reproducible performance across diverse experimental conditions. Its long-wavelength emission effectively mitigates background autofluorescence, thereby enhancing signal-to-noise ratios in complex biological matrices, including tissue samples. As the longest-wavelength fluorophore in the XFD series, XFD790 offers superior spectral separation from widely used far-red fluorophores such as iFluor(R) 647, XFD647, and allophycocyanin (APC), facilitating precise multicolor fluorescence analyses. Furthermore, its optical properties make it an excellent candidate for small animal in vivo imaging (SAIVI) and two-color western blot applications using the LI-COR(TM) Odyssey(TM) infrared imaging system. The DBCO derivative of XFD790 is a highly reactive cycloalkyne optimized for copper-free click chemistry (SPAAC, strain-promoted azide-alkyne cycloaddition). This derivative exhibits a significantly higher reaction rate with azides compared to other cyclooctynes and copper-catalyzed click reactions (CuAAC). Uniquely, DBCO does not react with tetrazines, allowing for its use in bioorthogonal reactions alongside trans-cyclooctenes and tetrazines. For applications where the presence of copper is problematic, XFD790 DBCO serves as an effective alternative to copper-dependent fluorescent alkynes.