XFD647 PEG4 DBCO

XFD647, manufactured by AAT Bioquest, is a bright far-red flurosecent dye structurally similar to Alexa Fluor(TM) 647 (Thermo Fisher). It is efficiently excited by the 594 nm or 633 nm laser lines and is compatible with RFP filters like Cy5, making it well-suited for applications such as fluorescence microscopy and flow cytometry. XFD647 exhibits excellent aqueous solubility and pH stability across a wide range (pH 4-10), ensuring robust signal reproducibility under diverse experimental conditions. The dye enables high-degree conjugation to biomolecules with minimal self-quenching, resulting in enhanced fluorescence intensity and signal stability. Additionally, its high fluorescence quantum yield and superior photostability facilitate the sensitive detection of low-abundance targets, enhancing precision and sensitivity in quantitative fluorescence-based assays. The DBCO derivative of XFD647 is a highly reactive cycloalkyne optimized for copper-free click chemistry (SPAAC, strain-promoted azide-alkyne cycloaddition). This derivative exhibits a significantly higher reaction rate with azides compared to other cyclooctynes and copper-catalyzed click reactions (CuAAC). Uniquely, DBCO does not react with tetrazines, allowing for its use in bioorthogonal reactions alongside trans-cyclooctenes and tetrazines. For applications where the presence of copper is problematic, XFD647 DBCO serves as an effective alternative to copper-dependent fluorescent alkynes.