XFD546 C5 maleimide

XFD546, manufactured by AAT Bioquest, is structurally similar to Alexa Fluor(TM) 546 (Thermo Fisher). It exhibits bright orange fluorescence and is readily excited by laser lines at 488 nm or 532 nm, making it highly suitable for applications such as fluorescence microscopy and flow cytometry. XFD546 demonstrates excellent aqueous solubility and pH-insensitivity over a broad range (pH 4-10), ensuring stable signal generation under varying experimental conditions. Additionally, the dye enables high molar ratio conjugation to proteins with minimal fluorescence quenching, facilitating the generation of brighter conjugates for enhanced detection sensitivity. With its high fluorescence quantum yield and superior photostability, XFD546 is particularly advantageous for detecting low-abundance biological targets, providing researchers with improved sensitivity and precision in quantitative fluorescence-based assays. The maleimide derivative of XFD546 is widely used for labeling biomolecules with free thiol (SH) groups, including antibodies, proteins, thiol-modified oligonucleotides, and low molecular weight ligands. Maleimides react readily with sulfhydryl groups, forming stable thio-ether bonds between the dye and the biomolecule, facilitating robust and reliable labeling for diverse experimental applications.