XFD532 Tetrazine

XFD532, manufactured by AAT Bioquest, is structurally similar to Alexa Fluor(TM) 532 (Thermo Fisher). It is a bright yellow-fluorescent dye with an excitation optimized for use with the 532 nm line of the frequency-doubled Nd:YAG laser. XFD532 demonstrates good aqueous solubility and pH-insensitivity over a broad pH range (pH 4-10), ensuring stable fluorescence generation under varying experimental conditions. This dye is particularly suited for multicolor fluorescence microscopy and flow cytometry, as well as advanced applications in super-resolution imaging techniques such as dSTORM. XFD532 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.