XFD532 PEG4 DBCO

XFD532, manufactured by AAT Bioquest, is structurally similar to Alexa Fluor(TM) 532 (Thermo Fisher). It is a bright yellow-fluorescent dye with an excitation optimized for use with the 532 nm line of the frequency-doubled Nd:YAG laser. The incorporation of a PEG4 linker enhances the aqueous solubility of XFD532, while its pH-insensitivity across a broad pH range (pH 4-10) ensures reliable and stable signal generation under diverse experimental conditions. XFD532 is particularly suited for multicolor fluorescence microscopy and flow cytometry, as well as advanced applications in super-resolution imaging techniques such as dSTORM. The DBCO derivative of XFD532 is a highly reactive cycloalkyne optimized for copper-free click chemistry (SPAAC, strain-promoted azide-alkyne cycloaddition). This derivative exhibits a significantly higher reaction rate with azides compared to other cyclooctynes and copper-catalyzed click reactions (CuAAC). Uniquely, DBCO does not react with tetrazines, allowing for its use in bioorthogonal reactions alongside trans-cyclooctenes and tetrazines. For applications where the presence of copper is problematic, XFD532 DBCO serves as an effective alternative to copper-dependent fluorescent alkynes.