XFD532 alkyne

XFD532, manufactured by AAT Bioquest, is structurally similar to Alexa Fluor(TM) 532 (Thermo Fisher). It is a bright yellow-fluorescent dye with an excitation optimized for use with the 532 nm line of the frequency-doubled Nd:YAG laser. XFD532 demonstrates good aqueous solubility and pH-insensitivity over a broad pH range (pH 4-10), ensuring stable fluorescence generation under varying experimental conditions. This dye is particularly suited for multicolor fluorescence microscopy and flow cytometry, as well as advanced applications in super-resolution imaging techniques such as dSTORM. The alkyne derivative of XFD532 is widely used for labeling azides on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-containing molecules.