XFD514 Tetrazine

XFD514, manufactured by AAT Bioquest, is a green-fluorescent dye structurally similar to Alexa Fluor(TM) 514 (Thermo Fisher). It is characterized by high photostability and exceptional brightness, making it highly suitable for advanced imaging and flow cytometry applications. The dye is water-soluble and remains pH-insensitive within a broad range (pH 4-10), ensuring consistent fluorescence performance across diverse experimental conditions. XFD514 offers robust and reliable labeling, enabling precise and reproducible results in fluorescence-based assays. It also serves as an effective replacement for rhodamine 6G in applications requiring enhanced performance. XFD514 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.