XFD488 aldehyde *Same Structure to Alexa Fluor(TM) 488 aldehyde*

Molecular weight: 707.66. Excitation (nm): 499. Emission (nm): 520. Extinction coefficient (cm-1 M-1): 73000. Quantum yield: 0.92. Correction factor (280 nm): 0.11. XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor(R) 488 (Alexa Fluor(R) is the trademark of ThermoFisher). XFD488 aldehyde contains the fluorophore of XFD488. It is a reactive green fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated XFD488 dye can be easily detected by a common fluorescence instrument under FITC channel.