XFD405 PEG4 DBCO

Product key features: Ex/Em: 401/421 nm Extinction coefficient: 35,000 cm-1M-1 Reactive Group: DBCO Copper-Free Bioorthogonal Labeling: Enables rapid and efficient azide conjugation via copper-free SPAAC Stable Fluorescence: Resists photobleaching, pH-insensitive from pH 4 to 10, and minimal quenching when conjugated to proteins Advanced PEG4 Spacer: Enhances solubility and minimizes steric hindrance for improved biocompatibility. Product description: XFD405, manufactured by AAT Bioquest, is a blue-fluorescent dye that is structurally identical to Alexa Fluor(TM) 405 (ThermoFisher). The incorporation of a PEG4 linker enhances its aqueous ability, and it is effectively excited by the 407 nm krypton laser line or the 408 nm violet laser diode, making it suitable for a range of fluorescence-based techniques. XFD405 is pH-insensitive across a wide range (pH 4 - 10) and exhibits minimal quenching when conjugated to proteins, ensuring consistent fluorescence signals in live-cell imaging. With an excitation maximum at 401 nm and emission at 422 nm, XFD405 is well-suited for multicolor flow cytometry and super-resolution microscopy (STORM), providing reliable performance in applications requiring distinct spectral separation and photostability. The DBCO derivative of XFD405 is a highly reactive cycloalkyne optimized for copper-free click chemistry (SPAAC, strain-promoted azide-alkyne cycloaddition). This derivative exhibits a significantly higher reaction rate with azides compared to other cyclooctynes and copper-catalyzed click reactions (CuAAC). Uniquely, DBCO does not react with tetrazines, allowing for its use in bioorthogonal reactions alongside trans-cyclooctenes and tetrazines. For applications where the presence of copper is problematic, XFD405 DBCO serves as an effective alternative to copper-dependent fluorescent alkynes.