UGO 647N NHS ester [equivalent to ATTO 647N NHS ester]

UGO 647N is a rhodamine-based fluorescent dye optimized for use in the red spectral region, with spectral characteristics similar to Cy5. It features high molar absorptivity, a strong fluorescence quantum yield, and excellent thermal and photostability. The dye is moderately hydrophilic and exhibits optimal excitation within the 625-660 nm range, making it compatible with the 647 nm line of Krypton-Ion lasers and the 650 nm line of diode lasers. UGO 647N maintains stable fluorescence across a broad pH range (pH 2-11), supporting its use in diverse experimental conditions. Upon conjugation to a substrate, it becomes cationic, carrying a net positive charge of +1. Unlike cyanine dyes, UGO 647N shows enhanced resistance to atmospheric ozone degradation, increasing its reliability for microarray applications. This dye is particularly effective for high-precision applications such as single-molecule detection, super-resolution microscopy techniques (e.g., SIM and STED), flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other biological assays. Chemical Properties Storage Upon receipt, store at -20°C in the dark. Protect from light and moisture. When stored as indicated, UGO NHS-esters are stable for at least 3 years. This product is for Research Use Only· Not for Human or Veterinary or Therapeutic Use CAS number 1199940-27-6 Formula C46H55ClN4O9 Molecular Weight (M+) 743 HPLC Purity >95% lambdaabs (nm) 646 epsilonmax (M-1 cm-1) 1.5x10^5 lambdafl (nm) 664 etafl 65% taufl (ns) 3.5 CF260 =epsilon260/epsilonmax 0.04 CF280 =epsilon280/epsilonmax 0.03