Protonex(TM) Red 600-Latex Bead Conjugate

Ex: 575 nm. Em: 597 nm. ProtonexT Red-latex bead conjugate demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of ProtonexT Red-latex bead conjugate. The fluorescence of ProtonexT Red-latex bead conjugate dramatically increases as pH decreases from neutral to the acidic, making it a robust tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the wash steps. ProtonexT Red-latex bead conjugate provides a powerful tool to study phagocytosis. ProtonexT Red-latex bead conjugate is non-fluorescent outside the cells, but fluoresce brightly red in acidic compartments (such as phagosomes, lysosomes and endosomes). This ProtonexT Red-latex bead conjugate can be also used for multiplexing cell functional analysis with green dyes such as GFP, Fluo-8, calcein, or FITC-labeled antibodies. ProtonexT Red has the spectral properties similar to those of Texas Red, making the common filter set of Texas Red readily available to the assays of ProtonexT Red.