Protonex(TM) Green 500-Latex Bead Conjugate

Ex: 445 nm. Em: 503 nm. Protonex(TM) Green-latex bead conjugate demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of Protonex(TM) Green-latex bead conjugate. The fluorescence of Protonex(TM) Green-latex bead conjugate dramatically increases as pH decreases from neutral to the acidic, making it a robust tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the wash steps. Protonex(TM) Green-latex bead conjugate provides a powerful tool to study phagocytosis. Protonex(TM) Green-latex bead conjugate is non-fluorescent outside the cells, but fluoresce brightly green in acidic compartments (such as phagosomes, lysosomes and endosomes). This Protonex(TM) Green-latex bead conjugate can be also used for multiplexing cell functional analysis with red dyes such as Texas Red-labeled antibodies. Protonex(TM) Green has the spectral properties similar to those of Fluorescein making the common filter set of Fluorescein readily available to the assays of Protonex(TM) Green.