Protonex(TM) Green 500

Ex: 443 nm. Em: 505 nm. Soluble in: DMSO. ProtonexT Green dye demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of ProtonexT Green dye. The fluorescence of ProtonexT Green dye increases as pH decreases from neutral to the acidic. The lack of fluorescence outside the cell eliminates the wash steps. ProtonexT Green dye provides a powerful tool to monitor acidic cell compartments such as endosomes and lysosomes. ProtonexT Green dye is non-fluorescent outside the cells, but fluoresces brightly green in acidic compartments (such as phagosomes, lysosomes and endosomes). This ProtonexT Green enables the specific detection of cellular acidic compartments with reduced signal variability and improved accuracy for imaging or flow applications. ProtonexT Green has the spectral properties similar to those of FITC, making the common filter set of FITC readily available to the assays of ProtonexT Green.