Phalloidin-iFluor(R) 660 Conjugate

Phalloidin iFluor(R) 660 is an F-actin-specific probe conjugated to the far red-fluorescent dye iFluor(R) 660. Phalloidin, a bicyclic peptide derived from Amanita phalloides ("death cap" mushroom), is widely used for the selective labeling of filamentous actin (F-actin) in fluorescence microscopy. The conjugation of phalloidin to iFluor(R) 660 retains its high binding affinity and specificity for F-actin, providing reliable and reproducible labeling across various biological systems. The iFluor(R) 660 fluorophore offers exceptional brightness and photostability, enabling prolonged imaging sessions with minimal photobleaching. Additionally, the probe exhibits negligible nonspecific binding, ensuring high-contrast visualization of actin filaments, even in complex biological environments. Phalloidin iFluor(R) 660 is optimized for high-resolution imaging and quantitative analysis of F-actin in diverse applications, including fixed tissue sections, cultured cells, and in vitro actin polymerization assays. Its compatibility with multiplex fluorescence imaging allows co-labeling with fluorescent proteins, quantum dots, iFluor(R) derivatives, and antibody-based detection systems.