PerCP-iFluor(R) 720

PerCP-iFluor(R) 720 is a tandem fluorophore specifically engineered for high-parameter flow cytometry applications. It consists of a peridinin chlorophyll protein (PerCP) donor covalently linked to an iFluor(R) 720 acceptor, enabling efficient energy transfer via Förster resonance energy transfer (FRET) upon excitation with a 488 nm blue laser. This results in a strong and well-defined emission peak near 747 nm, making it an ideal far-red reporter in multicolor panels. The spectral characteristics of PerCP-iFluor(R) 720 allow for minimal spillover into adjacent channels, such as PE and APC, facilitating cleaner resolution in complex staining panels. Compared to traditional PerCP tandems, PerCP-iFluor(R) 720 exhibits enhanced photostability and higher signal-to-noise ratios, improving sensitivity for the detection of low-abundance targets. Its consistent performance across fixation and permeabilization conditions makes it suitable for both surface and intracellular staining protocols. PerCP-iFluor(R) 720 can be covalently linked to proteins and antibodies using SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) or site-specific Buccutite(TM) MTA and Buccutite(TM) FOL conjugation chemistries. Conjugation to antibodies or streptavidin further expands its utility in immunophenotyping, cell subset discrimination, and high-dimensional cytometry.