PE-iFluor(TM) 647 Tandem

Excitation (nm): 569. Emission (nm): 666. Extinction coefficient (cm-1 M-1): 1960000. Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor(TM) 594 or iFluor(TM) 750). PE-iFluor(TM) 647 is a superior replacement to the commonly used PE-Cy5 tandem with significantly improved FRET efficiency and signal/background ratio. PE-iFluor(TM) 647 and PE-Cy5(R) and RPE-Alexa Fluor(R) 647 Tandem have almost identical spectra. AAT Bioquest also offers a unique preactivated PE-iFluor(TM) 647 to facilitate the PE- iFluor(TM) 647 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE- iFluor(TM) 647 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry.