PBFI

PBFI is a cell-impermeant fluorescent indicator for potassium ions, suitable for detection of physiological concentrations of K+ in the presence of other monovalent cations. PBFI is used to measure intracellular potassium (K+) fluxes in animal cells and in plant cells and vacuoles. The observation that intracellular K+ levels are a controlling factor in apoptotic cell death pathways, increased the interest for PBFI. PBFI has been used for detecting adrenoceptor-stimulated decreases of intracellular K+ concentration in astrocytes and neurons, evaluating the mediating effects of K+ depletion on monocytic cell necrosis, measuring intracellular K+ fluxes associated with apoptotic cell shrinkage, monitoring mitochondrial KATP channel activation, or detecting elevated intracellular K+ levels associated with HIV-induced cytopathology. Flow cytometric measurements using UV argon-ion laser excitation (351nm and 364nm) of PBFI indicate that K+ efflux induces shrinkage of apoptotic cells and is a trigger for caspase activation. Spectral data: lambdaex 336nm,, lambdaem 557nm. References: (1) P. Jezek, et al., J. Biol. Chem. 265, 10522 (1990) , (2) S.E. Kasner & M.B. Ganz, Am. J. Physiol. 262, F462 (1992) , (3) K. Venema, et al., Biochim. Biophys. Acta 1146, 87 (1993) , (4) R. Crossley, et al., J. Chem. Soc. Perk. Trans. 2, 513 (1994) , (5) K. Meuwis, Biophys. J. 68, 2469 (1995) , (6) H. Muyderman, et al., Neurochem. Int. 38, 269 (2001) , (7) A.L. Cook, et al., Cell Signal. 14, 1023 (2002) , (8) S.J. Halperin & J.P. Lynch, J. Exp. Bot. 54, 2035 (2003) , (9) D. Liu, et al., J. Neurochem. 86, 966 (2003) , (10) A.D. Costa, et al., Am. J. Physiol. Heart Circ. Physiol. 290, H406 (2006) , (11) P. Andersson, et al., Toxicol. In Vitro 20, 986 (2006) , (12) S. Jorgensen, et al., Methods Mol. Biol. 491, 257 (2008) , (13) R.W. Sabnis, Handbook of biological dyes and stains (2010) , (14) C.S. Arlehamn, et al., J. Biol. Chem. 285, 10508 (2010) Appearance: Brownish-yellow powder. Solubility: Soluble in methanol or DMSO. Identity: Declaration: Manufactured by Chemodex. Inchi Key: YOQMJMHTHWYNIO-UHFFFAOYSA-N Smiles: COC1=CC2=C(OC(C3=C(C(O)=O)C=C(C(O)=O)C=C3)=C2)C=C1N4CCOCCOCCN(C5=CC(OC(C6=C(C(O)=O)C=C(C(O)=O)C=C6)=C7)=C7C=C5OC)CCOCCOCC4