OxiVision(TM) Ratiometric Mitochondrial Lipid Peroxidation Sensor

A wide range of diseases is considered to result from mitochondrial oxidative damage that is associated with the lipid peroxidation of mitochondrial inner membranes. There are no specific methods to assess mitochondrial lipid peroxidation in live cells. To address this unmet need, AAT Bioquest has developed the OxiVisionT Ratiometric Mitochondrial Lipid Peroxidation Sensor, a fluorescent mitochondria-targeted probe that detects lipid peroxidation in live cells. OxiVisionT Ratiometric Mitochondrial Lipid Peroxidation Sensor enters cells rapidly, and selectively accumulates in mitochondria. It has a high specificity for the detection of mitochondrial lipid peroxidation. Mitochondrial lipid peroxidation results in a great change of fluorescence intensity ratio at 520 nm/590 nm, which can conveniently be monitored by fluorimetry, fluorescence microscopy or a fluorescence microplate reader. The fluorescence intensities are collected with FITC and Cy3/TRITC filter sets respectively at 520 nm/590 nm.