Nuclear Green(TM) Photo-Fixable DCS1 *5 mM DMSO Solution*

Excitation (nm): 502. Emission (nm): 527. Nuclear Green(TM) Photo-Fixable DCS1 is a unique fluorogenic, DNA-selective, and cell-impermeant dye for analyzing DNA content in dead, fixed, or apoptotic cells. It selectively stains cell membrane-compromised cells. It contains a DNA-binding fluorophore, thus readily binds to DNA upon interaction. Nuclear Green(TM) Photo-Fixable DCS1 has its green fluorescence significantly enhanced upon binding to DNA. In addition to its DNA-binding fluorophore, it contains a secondary DNA-photoreactive group. When needed, a moderate UV illumination (e.g., 350 nm) makes the DNA-bound dye readily react with DNA and makes the dye permanently conjugate with the DNA. It is optimized for surviving the cell fixation process better than common DNA dyes such as PI, DAPI, Hoechst, 7-AAD, DRAQ-5, DRAQ-7, or SYBR Green. It can be used in fluorescence imaging, microplate, and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of dead, fixed, or apoptotic cells with proper filter sets.