mFluor(TM) Violet 545 Styramide

Absorbance (nm): 398. Correction Factor (260 nm): 1.08. Correction Factor (280 nm): 0.496. Extinction coefficient (cm^-1 M^-1): 20000 (PBS). Excitation (nm): 393. Emission (nm): 543. Quantum yield: 0.15 (PBS). The Power Styramide(TM) Signal Amplification (PSA(TM)) system is a highly sensitive method for detecting low-abundant targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH). By utilizing bright and photostable mFluor(TM) dyes, Styramide(TM) conjugates deliver results of unparalleled sharpness and precision, surpassing the sensitivity of standard ICC/IHC/ISH methods by over 100 times, all the while reducing the consumption of primary antibodies. Like tyramide signal amplification (TSA), PSA(TM) leverages the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid sequence in situ. The enhanced reactivity of Styramide(TM) radicals over tyramide ensures faster, more robust labeling of the target, leading to fluorescence signals that are 10-50 times greater than those generated by tyramide (TSA) reagents. The mFluor(TM) Violet 545 Styramide uses the bright green fluorescent dye mFluor(TM) Violet 545 (Ex/Em = 393/543 nm) to label targets in situ. mFluor(TM) Violet 545 is well-excited by the 405 nm laser line and exhibits minimal crosstalk in complex multicolor analysis with blue and red fluorescent probes or other spectrally compatible Styramide conjugates and PSA(TM) Imaging Kits.