mFluor(TM) Violet 500 maleimide

mFluor(TM) Violet 500 Maleimide is an excellent building block that can be readily used for labeling biomolecules that have a free thiol (SH) group such as antibodies and thiol-modified oligos. mFluor(TM) Violet 500 dyes have fluorescence excitation and emission maxima of ~405 nm and ~500 nm respectively. These spectral characteristics make them an excellent replacement for Pacific Green(TM) (ThermoFisher) and BD Horizon(TM) V500 labeling dyes. mFluor(TM) Violet 500 Maleimide is reasonably stable and shows good reactivity and selectivity with free thiol group. mFluor(TM) Violet 500 Maleimide provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation. Under the same conditions, mFluor(TM) Violet 500 dye conjugates are significantly brighter than the corresponding bioconjugates of Pacific Green(TM) (ThermoFisher) and BD Horizon(TM) V500 with much stronger absorption, making the mFluor(TM) Violet 500 conjugates much more sensitive. AAT Bioquest's mFluor(TM) dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm).