mFluor(TM) UV375 maleimide

mFluor(TM) UV375 Maleimide is an excellent building block that can be readily used for labeling biomolecules that have a free thiol (SH) group such as antibodies and thiol-modified oligos. mFluor(TM) UV375 (MFUV375) dyes have fluorescence excitation and emission maxima of ~355 nm and ~375 nm respectively. These spectral characteristics make them an excellent alternative to BD Biosciences' BUV395. mFluor(TM) UV375 dyes are well excited by UV laser of 355 nm with emission centered around 375 nm, which perfectly matches the filter set of 379/28 nm used for BUV 395. In contrast to polymer-based BUV 395 dye, MFUV375 is a small organic molecule that can be readily conjugated to antibodies. It is dimmer than BUV395, might be used for brighter markers (such as CD4). AAT Bioquest's mFluor(TM) dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 355 nm, 405 nm, 488 nm and 633 nm).