iFluor(R) Ultra 488 succinimidyl ester

iFluor(R) Ultra 488 succinimidyl ester is a green fluorescent dye designed for labeling primary amines (R-NH2) on proteins, amine-modified oligonucleotides, and other amine-containing molecules. It is optimized for fluorescence microscopy and flow cytometry applications, providing high sensitivity and stability. Fluorescent dye-conjugated antibodies are essential tools for protein detection in a wide range of applications, including fluorescence microscopy, flow cytometry, Western blotting, and immunohistochemistry. The use of fluorescently labeled antibodies offers several advantages, such as enhanced sensitivity, multiplexing capability, and streamlined workflows. The iFluor(R) Ultra series represents an advanced generation of iFluor(R) dyes, specifically engineered for antibody labeling in fluorescence imaging and flow cytometry. iFluor(R) Ultra 488 exhibits superior photophysical properties compared to conventional green-fluorescent dyes, such as FITC, Oregon Green 488, and Alexa Fluor 488. Antibody conjugates prepared with iFluor(R) Ultra 488 display significantly greater fluorescence intensity under identical labeling conditions, potentially making it the brightest green-fluorescent dye for antibody conjugation. Additionally, its fluorescence remains stable across a broad pH range (4-10), ensuring consistent signal intensity in diverse experimental environments. The iFluor(R) Ultra 488 SE variant demonstrates high stability, reactivity, and selectivity for protein amine groups, facilitating efficient conjugation. With spectral properties and reactivity comparable to FITC, Oregon Green 488, and Alexa Fluor 488, iFluor(R) Ultra 488 serves as a high-performance alternative for fluorescence-based assays requiring superior signal brightness and stability.