Helixyte(TM) Green Alkyne

Helixyte(TM) Green is an excellent nucleic acid sensor that exhibits large fluorescence enhancement upon binding to dsDNA. It has the same spectral and DNA-binding properties as SYBR Green(R) (SYBR Green(R) is the trademark of ThermoFisher). AAT Bioquest's scientists developed Helixyte(TM) Green alkyne to enable the development of site-specific DNA probes. Helixyte(TM) Green alkyne is an excellent building block that readily reacts with azido-containing biological molecules such as azido-modified peptides, antibodies and oligos. Thus, it can be used to develop a variety of site-directed DNA probes. Helixyte(TM) Green is an excellent fluorogenic probe for quantifying DNAs with greatly improved sensitivity and selectivity. Helixyte(TM) Green is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biological procedures such as cDNA synthesis for library production and DNA fragment purification for subcloning, as well as diagnostic applications, such as quantitating DNA amplification products and primer extension assays.