EvaGreen(R), 2000X in DMSO

EvaGreen(R) Dye, 2000X  in DMSO is offered for customers with specialized applications requiring a concentrated dye solution.  Note: for most qPCR applications, we recommend our ready-to-use Forget-Me-Not(TM) EvaGreen(R) qPCR Master Mixes or EvaGreen(R) Dye, 20X  in water.  EvaGreen(R) dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR(R) Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen(R) dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen(R) dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR(R) Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer.  The unique properties of EvaGreen(R) dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR(R) Green I, EvaGreen(R) dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen(R) dye can be used at a much higher dye concentration than SYBR(R) Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.  EvaGreen(R) dye and applications are covered under granted and pending US and international patents.