DiYO(TM)-3

Ex: 612 nm. Em: 631 nm. Soluble in: Water. DiYO(TM)-3 is chemically equivalent to YOYO(R)-3 (YOYO(R) is the trademark of Invitrogen). DiYO(TM)-3 is a carbocyanine dimer with far-red fluorescence similar to Cy(R) 5 dyes. It is cell-impermeant and easily distinguished from fluorescein and rhodamine as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiYO(TM)-3 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Green(TM) LCS1 dye and cell-impermeant DiYO(R)-3 can be used to assess cell viability. DiYO(TM)-3 and Nuclear Green(TM) both have much greater extinction coefficients than that of DNA-bound propidium iodide.