Cal Red(TM) R525/650 AM

Cal Red R525/650 is weakly fluorescent, and once enters cells, the lipophilic AM blocking groups are cleaved by intracellular esterase, resulting in a negatively charged fluorescent dye retained well in cells with excitation close to 488 nm and two emissions at 525 nm and 650 nm. When cells are stimulated with a bioactive compound, the receptor initiates the release of intracellular calcium, which is chelated by Cal Red R525/650. The emission signal is increased at 525 nm and decreased at 650 nm when excited at 488 nm. The excitation and emission wavelength of Cal Red R525/650 are compatible with common filter sets with minimal damage to cells. Ex: 492 nm. Em: 650 nm.