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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
Cay15922-1 | 1 mg | - |
6 - 10 business days* |
171.00€
|
|||
Cay15922-5 | 5 mg | - |
6 - 10 business days* |
771.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
BCECF AM ester is a widely-used fluorescent indicator for estimating intracellular pH. This... more
Product information "BCECF AM"
BCECF AM ester is a widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF AM can also be used to investigate intracellular changes in other ions, including potassium.Formal Name: 3',6'-bis[(acetyloxy)methoxy]-5(or 6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoic acid, 2',7'-bis[(acetyloxy)methyl] ester. Synonyms: BCECF Acetoxymethyl ester. Molecular Formula: C42H40O21. Formula Weight: 880.7. Purity: >95%. Emission: 535 nm. Excitation: 440, 490 nm. Formulation: (Request formulation change), An oil. Solubility: Acetonitrile: Soluble, DMF: Soluble, DMSO: Soluble. SMILES: O=C(C)OCOC1=C(CCC(OCOC(C)=O)=O)C=C(C2(C(C=CC(C(OCOC(C)=O)=O)=C3)=C3C(O2)=O)C(C(O4)=C5)=CC(CCC(OCOC(C)=O)=O)=C5OCOC(C)=O)C4=C1.O=C(C)OCOC6=C(CCC(OCOC(C)=O)=O)C=C(C7(C(C=C(C(OCOC(C)=O)=O)C=C8)=C8C(O7)=O)C(C(O9)=C%10)=CC(CCC(OCOC(C)=O)=O)=C%10OCOC(C)=O)C9=C6. InChi Code: InChI=1S/2C42H40O21/c1-22(43)52-17-57-34-15-36-32(13-27(34)7-10-38(48)59-19-54-24(3)45)42(31-9-6-29(12-30(31)41(51)63-42)40(50)61-21-56-26(5)47)33-14-28(8-11-39(49)60-20-55-25(4)46)35(16-37(33)62-36)58-18-53-23(2)44,1-22(43)52-17-57-34-15-36-32(12-27(34)7-10-38(48)59-19-54-24(3)45)42(31-14-29(6-9-30(31)41(51)63-42)40(50)61-21-56-26(5)47)33-13-28(8-11-39(49)60-20-55-25(4)46)35(16-37(33)62-36)58-18-53-23(2)44/h2*6,9,12-16H,7-8,10-11,17-21H2,1-5H3. InChi Key: JBGGWLORWOIHKM-UHFFFAOYSA-N.
Keywords: | BCECF Acetoxymethyl ester, 3',6'-bis[(acetyloxy)methoxy]-5(or 6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoic acid, 2',7'-bis[(acetyloxy)methyl] ester |
Supplier: | Cayman Chemical |
Supplier-Nr: | 15922 |
Properties
Application: | Fluorescent intracellular pH indicator |
MW: | 880.7 D |
Formula: | C42H40O21 |
Purity: | >95% |
Format: | Solid |
Database Information
Handling & Safety
Storage: | -20°C |
Shipping: | +20°C (International: -20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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