Ac-LEHD-AFC (trifluoroacetate salt)

Ac-LEHD-AFC is a fluorogenic substrate that can be cleaved by caspase-4, -5, and -9. Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.Formal Name: N-acetyl-L-leucyl-L-alpha-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-alpha-asparagine, trifluoroacetate salt. Synonyms: N-Acetyl-Leu-Glu-His-Asp-7-amino-4-Trifluoromethylcoumarin, Caspase-9 substrate (Fluorogenic). Molecular Formula: C33H38F3N7O11 . XCF3COOH. Formula Weight: 765.7. Purity: >95%. Emission: 505 nm. Excitation: 400 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 5 mg/ml, DMSO: 2 mg/ml, Ethanol: 2 mg/ml, PBS (pH 7.2): 1 mg/ml. lambdamax: 211, 340 nm. SMILES: O=C([C@H](CC1=CNC=N1)NC([C@H](CCC(O)=O)NC([C@H](CC(C)C)NC(C)=O)=O)=O)N[C@H](C(NC2=CC=C3C(C(F)(F)F)=CC(OC3=C2)=O)=O)CC(O)=O.FC(F)(C(O)=O)F. InChi Code: InChI=1S/C33H38F3N7O11.C2HF3O2/c1-15(2)8-22(39-16(3)44)31(52)41-21(6-7-26(45)46)29(50)42-23(9-18-13-37-14-38-18)32(53)43-24(12-27(47)48)30(51)40-17-4-5-19-20(33(34,35)36)11-28(49)54-25(19)10-17,3-2(4,5)1(6)7/h4-5,10-11,13-15,21-24H,6-9,12H2,1-3H3,(H,37,38)(H,39,44)(H,40,51)(H,41,52)(H,42,50)(H,43,53)(H,45,46)(H,47,48),(H,6,7)/t21-,22-,23-,24-,/m0./s1. InChi Key: IKHXNWAISFEPLL-JPIABUGISA-N.