AATOM(TM) 680 maleimide

Product key features: Ex/Em: 699/715 nm Extinction coefficient: 120,000 cm^(-1)M^(-1) Reactive group: maleimide Versatile Conjugation: Efficiently labels thiol groups on proteins, antibodies, and oligonucleotides with covalent thioether bonds High Quantum Yield & Stability: Provides bright fluorescence with high photostability and thermal resilience Zwitterionic: Minimizes non-specific binding to biological surfaces, reducing background noise in live-cell imaging and super-resolution microscopy. Product description: AATOM(TM) 680 is a far-red fluorescent dye characterized by its strong absorption, high photo and thermal stability, and good aqueous solubility. It is optimally excited within the 645-695 nm wavelength range, which aligns with the 670 nm line of diode laser and the 676 nm line of Krypton-Ion laser. As a zwitterionic compound, AATOM(TM) 680 remains electrically neutral when conjugated to biomolecules or other substrates. Its strong electron-accepting properties result in efficient fluorescence quenching by electron donors such as guanine and tryptophan. These properties make AATOM(TM) 680 ideal for precise applications including single-molecule detection and super-resolution microscopy techniques like PALM, dSTORM, and STED. Furthermore, AATOM(TM) 680 is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a variety of other biological assays, making it a versatile tool in advanced fluorescence-based research. The maleimide derivative of AATOM(TM) 680 is widely used for labeling biomolecules with free thiol (SH) groups, including antibodies, proteins, thiol-modified oligonucleotides, and low molecular weight ligands. Maleimides react readily with sulfhydryl groups, forming stable thio-ether bonds between the dye and the biomolecule, facilitating robust and reliable labeling for diverse experimental applications.