AATOM(TM) 514 maleimide

Correction Factor (260 nm): 0.21 Correction Factor (280 nm): 0.08 Extinction coefficient (cm^-1 M^-1): 115,000 Excitation (nm): 305 Emission (nm): 531 Quantum yield: 0.85. AATOM(TM) 514 is a rhodamine-based fluorescent labeling dye known for its favorable spectral qualities. With a high molar extinction coefficient, a fluorescence quantum yield of 0.85, high photo-stability, and excellent water solubility, it is an ideal choice for single-molecule detection, high-resolution microscopy (e.g., SIM and STED), flow cytometry, and fluorescence in-situ hybridization (FISH). It exhibits its highest fluorescence efficiency when excited within the range of 510-535 nm, making it a perfect match for the 514 nm line of an argon-ion laser. AATOM(TM) 514 maleimide is used for labeling thiol-containing molecules, such as proteins, antibodies, ligands, and oligonucleotide thiophosphates. Solvent: DMSO.