Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an... read more »
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Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

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TMRM (Tetramethylrhodamine methyl ester)
TMRM (Tetramethylrhodamine methyl ester)

Item number: ABD-22221

Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. TMRE is slightly more hydrophobic than TMRM.
Application: Mitochondrial membrane potential assays
CAS 115532-50-8
143.00€ *
Review
CFDA-SE
CFDA-SE

Item number: Cay14456-10

CFDA-SE is a stable, cell-permeable dye that consists of a fluorescein molecule containing two acetate moieties and a succinimidyl ester (SE) functional group. Upon diffusion into the intracellular environment, the acetate groups are cleaved by cellular esterases leaving CFSE (Cay-16802), which is fluorescent and...
Keywords: 5(6)-Carboxyfluorescein diacetate succinimidyl ester, 5(6)-CFDA N-succinmidyl ester,...
Application: Cell proliferation assays
CAS 150347-59-4
MW: 557.5 D
From 119.00€ *
Review
BODIPY 493/503
BODIPY 493/503

Item number: Cay25892-10

BODIPY 493/503 is a lipophilic fluorescent probe that localizes to polar lipids and can be used to label cellular neutral lipid contents, particularly those localized to lipid droplets, in live and fixed cells. BODIPY 493/503 is compatible with epifluorescent, confocal, and two-photon microscopy, as well as flow...
Keywords: (T-4)-[2-[1-(3,5-dimethyl-2H-pyrrol-2-ylidene-kappaN)ethyl]-3,5-dimethyl-1H-pyrrolato-kappaN]difluoro-boron
Application: Lipophilic fluorescent probe, FC, epifluorescent, confocal, two-photon microscopy
CAS 121207-31-6
MW: 262.1 D
From 54.00€ *
Review
JC-1 (5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide)
JC-1...

Item number: ABD-22200

Widely used for determining mitochondrial membrane potential with flow cytometry. It is capable of entering selectively into mitochondria, and changes reversibly its color from green to orange as membrane potentials increase.
Application: Mitochondrial membrane potential assays
CAS 3520-43-2
From 145.00€ *
Review
Cal-630 (TM) AM
Cal-630 (TM) AM

Item number: ABD-20531

Ex: 608 nm. Em: 626 nm. Soluble in: DMSO. Cal-630(TM) has been developed to improve x-Rhod-1 cell loading and calcium response while maintaining the spectral wavelength of x-Rhod-1, making it compatible with Texas Red(R) filter set. In CHO and HEK cells Cal-630(TM) AM has cellular calcium response that is much more...
MW: 1342.94 D
From 434.00€ *
Review
SBD-F
SBD-F

Item number: CDX-F0002-M250

Powder. Soluble in water or 1M ammonium hydroxide (50 mg/ml). Fluorescent probe for thiols widely used in pre-column labeling of biological thiols for HPLC.
Keywords: 7-Fluorobenzofurazan-4-sulfonic acid ammonium salt
Application: Thiol labeling, Fluorescent thiol probe, HPLC
CAS 84806-27-9
MW: 235,19 D
From 78.00€ *
Review
Hydroethidine (Dihydroethidium)
Hydroethidine (Dihydroethidium)

Item number: ABD-15200

Hydroethidine operates effectively as a probe for measurement of reactive oxygen species. The dye enters cells freely and is dehydrogenated to ethidium bromide.
Application: ROS assays
CAS 38483-26-0
145.00€ *
Review
Phalloidin-iFluor(TM) 555 Conjugate
Phalloidin-iFluor(TM) 555 Conjugate

Item number: ABD-23119

This orange fluorescent phalloidin conjugate (equivalent to Alexa Fluor(R) 555-labeled phalloidin) selectively binds to F-actins. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections,...
Application: Labeling
MW: 2,10 kD
218.00€ *
Review
OG488 BAPTA-1, AM [similar to Oregon Green(R) 488 BAPTA-1, AM] *Cell permeant*
OG488 BAPTA-1, AM [similar to Oregon Green(R) 488...

Item number: ABD-20507

Oregon Green(R) is a trademark of Life Technologies.
Keywords: Oregon Green(R) is a trademark of Life Technologies. This product is equivalent to analog488 BAPTA-1 (20507) on the AAT...
291.00€ *
Review
FMOC-Lys(5-FAM)-OH
FMOC-Lys(5-FAM)-OH

Item number: ABD-5043

A building block for in-sequence Lys labeling by FAM.
Application: Building block
CAS 1242933-88-5
145.00€ *
Review
Cal-520-Dextran Conjugate *MW 10,000*
Cal-520-Dextran Conjugate *MW 10,000*

Item number: ABD-20601

Ex: 492 nm. Em: 514 nm. Soluble in: Water. Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow...
Application: IF, FC, Calcium detection
434.00€ *
Review
Fluo-3, pentapotassium salt
Fluo-3, pentapotassium salt

Item number: ABD-21017

Fluo-3 is essentially nonfluorescent unless bound to Ca2+ and exhibits a quantum yield at saturating Ca2+ of 0.14 and a Kd for Ca2+ of 390 nM.
Application: Calcium indicator
218.00€ *
Review
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