Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an... read more »
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Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

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Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)*
Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)*

Item number: ABD-17017

The biotin-modified deoxyuridine 5'-triphosphates are widely used for a variety of non-radioactive DNA labeling reactions including nick translation, random prime labeling, cDNA labeling and 3'-end labeling. The biotinylated probes have been shown to hybridize to homologous nucleic acid at the same rate and to the...
Application: Labeling
CAS 136632-31-0
MW: 1,01 kD
145.00€ *
Review
BrdU (5-Bromo-2'-deoxyuridine)
BrdU (5-Bromo-2'-deoxyuridine)

Item number: ABD-17030

BrdU is a commonly used in the detection of proliferating cells in living tissues. It works by substituting for thymidine during DNA replication and incorporating itself into the newly synthesized DNA (during S-phase).
Application: Cell proliferation assays
CAS 59-14-3
114.00€ *
Review
Zinquin *UltraPure grade*
Zinquin *UltraPure grade*

Item number: ABD-21260

Zinquin AM ester is a zinc-sensitive, cell-impermeable fluorescent probe. Zinc is the second most abundant transition metal in the body and it is essential as catalytic, structural and regulatory ion. Zinc ions are involved in homeostasis, immune responses, oxidative stress, apoptosis and aging. Zinc has been...
Application: Zinc monitoring
CAS 151606-29-0
145.00€ *
Review
DAPI Dilactate
DAPI Dilactate

Item number: ABD-17509

DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA...
Application: IF
145.00€ *
Review
Fluorescein, disodium salt *Fluorescence reference standard*
Fluorescein, disodium salt *Fluorescence reference standard*

Item number: ABD-2

Fluorescein, disodium salt is water-soluble version of fluorescein.
Application: Labeling
114.00€ *
Review
DNP-X acid, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester)
DNP-X acid, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid,...

Item number: ABD-2021

An amine-reactive building block for developing a probe that can be recognized by anti-DNP antibodies, Also an excellent amine-reactive FRET quencher paired with Trp or Tyr.
Application: Labeling
CAS 82321-04-8
145.00€ *
Review
CDCFDA, SE (5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester) *Mixed isomer
CDCFDA, SE (5-(and-6)-Carboxy-2',7'-dichlorofluorescein...

Item number: ABD-22026

5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester, is an amine reactive FDA derivative that can be used to prepare a variety of FDA conjugates.
Application: Cell viability assays
145.00€ *
Review
DiOC5(3) iodide (3,3-Dipentyloxacarbocyanine iodide)
DiOC5(3) iodide (3,3-Dipentyloxacarbocyanine iodide)

Item number: ABD-22045

Lipophilic fluorescent stain. DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells.
Application: Lipophilic fluorescent stain
114.00€ *
Review
CytoCalcein Violet 450, AM *Excited at 405 nm*
CytoCalcein Violet 450, AM *Excited at 405 nm*

Item number: ABD-22012

Calcein Violet 450, AM is designed for labeling live cells as calcein AM. Compared to calcein blue, Calcein Violet 460, AM is brighter, and can be better excited with 405 nm laser line.
Application: Cell viability assays
291.00€ *
Review
Phalloidin-iFluor(TM) 532 Conjugate
Phalloidin-iFluor(TM) 532 Conjugate

Item number: ABD-23117

This orange fluorescent phalloidin conjugate (equivalent to Alexa Fluor(R) 532-labeled phalloidin) selectively binds to F-actins. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections,...
Application: Labeling
MW: 1,80 kD
218.00€ *
Review
Biotin Azide
Biotin Azide

Item number: ABD-3020

Soluble in: DMSO. Biotin and biotin derivatives bind with high affinity to both avidins and streptavidins. Biotin azide can be readily conjugated to many biomolecules through the well-known click chemistry.
Application: Biotin conjugation
MW: 312,29 D
128.00€ *
Review
Tide Fluor(TM) 3WS acid (TF3 acid) *Superior replacement to Cy3*
Tide Fluor(TM) 3WS acid (TF3 acid) *Superior replacement...

Item number: ABD-2345

Tide Fluor(TM) 3WS (TF3WS) family has the spectral properties essentially identical to those of Cy3. Compared to Cy3 probes TF3WS family has better water solubility. It has been used to label the hydrophobic peptides that hasve poor water solubility. Additionally their fluorescence is pH-independent from pH 3 to 11....
Application: Labeling
MW: 838,89 D
434.00€ *
Review
54 from 269 pages