Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an... read more »
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Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

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6-TAMRA, SE (6-Carboxytetramethylrhodamine, succinimidyl ester) *Single isomer*
6-TAMRA, SE (6-Carboxytetramethylrhodamine, succinimidyl...

Item number: ABD-376

The single TAMRA isomers are increasingly preferred for labeling peptides and nucleotides because they give better resolution in HPLC purification that is often required in the conjugation processes.
Application: Labeling
CAS 150810-69-8
From 151.00€ *
Review
Indo-1 pentapotassium salt
Indo-1 pentapotassium salt

Item number: CDX-I0018-M002

Off-white solid. Soluble in water. Fluorescent calcium chelator and indicator with an unusually large shift in fluorescence emission from 480 nm to 400 nm on binding of calcium. Useful for intracellular application.
Application: Fluorescent calcium chelator / indicator
CAS 132319-56-3
MW: 840,05 D
From 230.00€ *
Review
FAM Dye qPCR Calibration Solution *10,000X*
FAM Dye qPCR Calibration Solution *10,000X*

Item number: ABD-67032

Correction Factor (260 nm): 0.32 Correction Factor (280 nm): 0.178 Extinction coefficient (cm^-1 M^-1): 83000 Excitation (nm): 493 Emission (nm): 517. Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal. Since instruments can differ in fluorescence detection...
Application: Real-time thermal cycler calibration
395.00€ *
Review
Cy5 Dye qPCR Calibration Solution *10,000X*
Cy5 Dye qPCR Calibration Solution *10,000X*

Item number: ABD-67033

Correction Factor (260 nm): 0.02 Correction Factor (280 nm): 0.03 Correction Factor (482 nm): 0.009 Correction Factor (565 nm): 0.09 Extinction coefficient (cm^-1 M^-1): 250000 (Ethanol) Excitation (nm): 651 Emission (nm): 670 Quantum yield: 0.27 (PBS), 0.4 (Ethanol). Real-time thermal cyclers require calibration to...
Application: Real-time thermal cycler calibration
395.00€ *
Review
ROX Dye qPCR Calibration Solution *10,000X*
ROX Dye qPCR Calibration Solution *10,000X*

Item number: ABD-67034

Excitation (nm): 578 Emission (nm): 595. Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal. Since instruments can differ in fluorescence detection sensitivity, the concentrations needed to calibrate any dye, such as ROX, will depend on the device used. The AAT...
Application: Real-time thermal cycler calibration
395.00€ *
Review
Hoechst 33342 amine
Hoechst 33342 amine

Item number: ABD-17671

Excitation (nm): 352. Emission (nm): 454. Hoechst 33342 amine is an excellent building block for developing site-specific nucleic acid probes. It can be readily conjugated to carbonyl-containing biomolecules (such as antibodies, peptides and oligos). Hoechst 33342 is a DNA-specific dye that binds to the minor groove...
Application: Site-specific nucleic acid probe building block, carbonyl-reactive
MW: 495.63 D
249.00€ *
Review
DAPI, 10mg/mL in H2O
DAPI, 10mg/mL in H2O

Item number: BOT-40043

DAPI (4',6-Diamidino-2-Phenylindole) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Because...
CAS 28718-91-4
152.00€ *
Review
LipidSpot(TM) 610 Lipid Droplet Stain
LipidSpot(TM) 610 Lipid Droplet Stain

Item number: BOT-70069-T

LipidSpot(TM) 610 dyes rapidly stains lipid droplets with red/far-red fluorescence for detection in the Texas Red(R) or Cy(R)5 channels. For live or fixed cells, no wash step required. Note: due to its broad emission, we do not recommend pairing LipidSpot(TM) 610 with other red or far-red probes.
Keywords: lipid droplets, adipocytes, LipidTox
From 108.00€ *
Review
CF(R)633-Dextran 70,000 MW, Anionic and Fixable
CF(R)633-Dextran 70,000 MW, Anionic and Fixable

Item number: BOT-80141

This CF(R) dye labeled dextran has molecular weight of 70,000 and a formaldehyde-fixable amine group, for use as a fluid-phase marker to study cell permeability, endocytosis, or biomolecule delivery. Far-red fluorescent CF(R)633 dye has excitation/emission at 630/650 nm.
189.00€ *
Review
AATOM(TM) 655 maleimide
AATOM(TM) 655 maleimide

Item number: ABD-70282

Product key features: Ex/Em: 661/679 nm Extinction coefficient: 125,000 cm^(-1)M^(-1) Reactive group: maleimide Versatile Conjugation: Efficiently labels thiol groups on proteins, antibodies, and oligonucleotides with covalent thioether bonds High Quantum Yield & Stability: Provides bright fluorescence with high...
Application: Far-red fluorescent dye, strong absorption, highly photo/thermal stable/ozone resistant/water soluble
145.00€ *
Review
Fluorescein *Fluorescence reference standard*
Fluorescein *Fluorescence reference standard*

Item number: ABD-1

Fluorescein is a fluorophore widely used in microscopy, forensics and serology to detect latent blood stains. Fluorescein has an absorption maximum at 490 nm and emission maximum of 514 nm (in water).
Application: Labeling
114.00€ *
Review
iFluor(TM) 405 succinimidyl ester
iFluor(TM) 405 succinimidyl ester

Item number: ABD-1021

AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor(TM) 405 dyes have...
Application: Labeling
291.00€ *
Review
48 from 269 pages