Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an... read more »
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Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

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Rhodamine 110 *Fluorescence reference standard*
Rhodamine 110 *Fluorescence reference standard*

Item number: ABD-86

This compound is the precursor for a variety of R110-based enzyme substrates. Compared to AMC and AFC enzyme substrates, fluorogenic R110 substrates are generally more sensitive,
Application: Labeling
CAS 13558-31-1
218.00€ *
Review
CEC (3-Cyano-7-ethoxycoumarin)
CEC (3-Cyano-7-ethoxycoumarin)

Item number: ABD-15001

This fluorogenic coumarin ether is widely used for monitoring P450 activities in cell extracts and solutions.
Application: P450 assays
CAS 117620-77-6
114.00€ *
Review
Thiazole orange *10 mM in DMSO*
Thiazole orange *10 mM in DMSO*

Item number: ABD-17519

A fluorescent DNA-binding dye for reticulocyte analysis. Also useful for Plasmodium species analysis.
Application: Nucleic acid staining
114.00€ *
Review
MESG *Phosphate assay reagent*
MESG *Phosphate assay reagent*

Item number: ABD-21600

In the presence of inorganic phosphate MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase (EC 2.4.2.1) with absorption wavelength shift to red.
Application: Phosphatase quantification
261.00€ *
Review
DiA (4-(4-(Dihexadecylamino)styryl)-N-methylpyridinium iodide)
DiA (4-(4-(Dihexadecylamino)styryl)-N-methylpyridinium...

Item number: ABD-22030

Lipophilic fluorescent stain for labeling membranes and other hydrophobic structures. This dye diffuses laterally within the cellular plasma membranes, resulting in even staining of the entire cell at its optimal concentrations.
Application: Lipophilic fluorescent stain
114.00€ *
Review
Pentafluorobenzenesulfonyl fluorescein
Pentafluorobenzenesulfonyl fluorescein

Item number: Cay10005983-1

Reactive oxygen species (ROS) play important roles in the initiation and progression of many disease processes. Most fluorescent probes for ROS detection, such as 2,7-dichlorofluorescein (DCFH), function by an oxidative mechanism and are useful for total oxidant detection but are not selective for hydrogen peroxide...
Keywords: pentafluorobenzenesulfonic acid, 6'-hydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl ester
Application: H2O2 probe
CAS 728912-45-6
MW: 562.4 D
From 65.00€ *
Review
Biotin-X-NHS
Biotin-X-NHS

Item number: Cay13316-100

N-Hydroxysuccinimide (NHS) activates carboxylic acid groups (on biotin) to facilitate coupling reactions. Biotin-X-NHS is a compound used to attach biotin to primary amines under alkaline conditions (pH~8-9). For example, lysines on the surface of proteins, including antibodies, are ideal targets for biotinylation...
Keywords: 6-[[5-[(3aS,4S,6aR)-hexhydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]-2,5-dioxo-hexanoic acid,...
Application: Biotinylation
CAS 72040-63-2
MW: 454.5 D
From 180.00€ *
Review
Fluorescein-5-maleimide
Fluorescein-5-maleimide

Item number: Cay16383-25

Fluorescein-5-maleimide is an activated fluorescent molecule (excitation: 494 nm, emission: 519 nm) that is used to easily conjugate fluorescein to proteins. The maleimide group reacts optimally with sulfhydryl groups on cysteine side chains at pH 7, forming a stable thioether bond. The compound can be used to label...
Keywords: 5-Maleimido-fluorescein, 5-MF, 1-(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-1H-pyrrole-2,5-dione
Application: Fluorescent protein labeling
CAS 75350-46-8
MW: 427.4 D
From 69.00€ *
Review
Rhodamine 110 chloride
Rhodamine 110 chloride

Item number: CDX-R0016-G001

Red to red-brown crystalline or powder. Soluble in ethanol, methanol, DMSO or DMF. Slightly soluble in water. Fluorescent dye. Fluorophore standard that is widely used in enzyme assay systems. Precursor for a variety of R110-based enzyme substrates for flow cytometric measurements. Used as a calibration standard for...
Keywords: RH110, Rhodamine 560 Chloride, Rhodamine N, 3,6-Diamino-9-(2-carboxyphenyl)xanthylium chloride
Application: Fluorescent dye, fluorophore standard
CAS 13558-31-1
MW: 366,8 D
From 77.00€ *
Review
DAF-FM DA
DAF-FM DA

Item number: CDX-A0024-M001

Solid. Soluble in DMSO. DAF-FM diacetate is an important reagent for quantification of low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using...
Keywords: 3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein diacetate
Application: Nitric oxide quantitation
CAS 254109-22-3
MW: 496,42 D
From 443.00€ *
Review
DAF-2 DA
DAF-2 DA

Item number: CDX-D0085-M001

Powder. Soluble in DMSO. DAF-2 DA is probably the most successful indicator for nitric oxide. As with other fluorescein diacetates, DAF-2 diacetate is membrane permeant and is deacetylated by intracellular esterases to 4,5-diaminofluorescein (DAF-2). Can be used for the fluorimetric detection of nitric oxide and in...
Keywords: 4,5-Diaminofluorescein diacetate
Application: Nitric oxide indicator
CAS 205391-02-2
MW: 446,41 D
From 321.00€ *
Review
Fluo-8(R), sodium salt
Fluo-8(R), sodium salt

Item number: ABD-21088

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and...
Application: Calcium indicator
From 291.00€ *
Review
42 from 269 pages