Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an... read more »
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Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

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Quest Fluo-8L(TM), AM
Quest Fluo-8L(TM), AM

Item number: ABD-21097

Fluorescent probe that show spectral responses upon binding Ca2+. Superior compared to Fluo-3 AM and Fluo-4 AM. (Quest Fluo-8: Kd = 389 nM, Quest Fluo-8H: Kd = 232 nM, Quest Fluo-8L: Kd = 1.86 µM).
Application: Calcium indicator
From 291.00€ *
Review
BrdUTP (5-Bromo-2?-deoxyuridine 5?-triphosphate) *10 mM in TE Buffer*
BrdUTP (5-Bromo-2?-deoxyuridine 5?-triphosphate) *10 mM...

Item number: ABD-17031

BrdUTP can be used for incorporating into DNA for the subsequent detection with anti-BrdU antibodies. BrdUTP incorporation into DNA is also a tool for random-mutation introduction.
Application: Labeling
CAS 102212-99-7
291.00€ *
Review
Indo-1, pentapotassium salt
Indo-1, pentapotassium salt

Item number: ABD-21040

Indo-1 is the preferred dye for flow cytometry. Indo-1 AM is cell-permeable version of Indo-1.
Application: Ratiometric fluorescent calcium indicator acetoxy-methyl ester, FC
CAS 132319-56-3
145.00€ *
Review
Rhodamine B, hexyl ester, perchlorate
Rhodamine B, hexyl ester, perchlorate

Item number: ABD-22211

Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells.
Application: Mitochondrial function assays
CAS 877933-92-1
114.00€ *
Review
CytoTell(TM) Red
CytoTell(TM) Red

Item number: ABD-22255

Ex: 628 nm. Em: 643 nm. Soluble in: DMSO. Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations. Among all the existing fluorescent dyes CFSE is the preferred cell proliferation indicator that is widely used for live cell analysis. However, it is impossible...
Application: Cell-based assays, fluorescent cell labeling, FC
MW: ~600 D
From 145.00€ *
Review
EDANS acid (5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid)
EDANS acid (5-((2-Aminoethyl)amino)naphthalene-1-sulfonic...

Item number: ABD-610

One of the most popular donors for developing FRET-based nucleic acid probes (such as Molecular Beacons) and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive.
Application: Labeling
CAS 50402-56-7
145.00€ *
Review
CytoTrace Green(TM) CMFDA
CytoTrace Green(TM) CMFDA

Item number: ABD-22017

Ex: 494 nm. Em: 521 nm. Soluble in: DMSO. CytoTrace GreenT fluorescent probe retains the spectral properties of FITC, thus its signal can be typically monitored using fluorescence microscopy or flow cytometry. It freely passes through cell membranes and is converted to cell-impermeant products upon reactions with...
Application: IF, FC, long-term cell tracer
MW: 464,86 D
291.00€ *
Review
DiOC2(3) iodide (3,3-Diethyloxacarbocyanine iodide)
DiOC2(3) iodide (3,3-Diethyloxacarbocyanine iodide)

Item number: ABD-22038

Lipophilic fluorescent stain. DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells.
Application: Lipophilic fluorescent stain
114.00€ *
Review
Trypan Blue, sodium salt *UltraPure grade* *Purified to eliminate fluorescent impurities*
Trypan Blue, sodium salt *UltraPure grade* *Purified to...

Item number: ABD-2452

Trypan blue, a diazo dye is a vital stain that colors dead tissues or cells blue. Live cells or tissues with intact cell membranes are not be colored.
Application: Cell staining
291.00€ *
Review
DBCO-Cy3
DBCO-Cy3

Item number: ABD-920

Ex: 555 nm. Em: 565 nm. Soluble in: DMSO. This azadibenzocyclooctyne-cyanine dye derivative is a versatile labeling reagent for detection of azide containing molecules or compounds. Cyclooctynes are useful in strain-promoted copper-free azide-alkyne cycloaddition reactions. This dibenzocyclooctyne will react with...
Application: Labeling probe, Classic labeling dye, Cyanine
MW: 1185,56 D
145.00€ *
Review
Rhodamine 123 (chloride)
Rhodamine 123 (chloride)

Item number: Cay16672-5

Rhodamine 123 is a membrane-permeable cationic dye that is readily accumulated within living cells. It is a substrate for the efflux pump P-glycoprotein (P-gp, also known as multidrug resistance protein 1 and ABCB1) and is rapidly exported from cells with functional P-gp. As P-gp is expressed on a population of stem...
Keywords: R-22420, R-302, RH123, 3,6-diamino-9-[2-(methoxycarbonyl)phenyl]-xanthylium, monochloride
Application: Membrane-permeable cationic dye, P-glycoprotein substrate, mitochondrial stain
CAS 62669-70-9
MW: 380.8 D
From 51.00€ *
Review
JC-10 (high purity)
JC-10 (high purity)

Item number: AG-CR1-3600-M005

Deep blue to violet solid. Soluble in water or DMSO. JC-10 is superior analog of JC-1, useful for determining mitochondrial membrane potential (MMP) in cells by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-10 is a cationic, lipophilic dye that accumulates in mitochondria of...
Keywords: Enhanced JC-1
Application: Mitochondrial function assays
MW: 600 D
From 83.00€ *
Review
40 from 269 pages