Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an... read more »
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Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

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Oxazine 1
Oxazine 1

Item number: ABD-89

Oxazine 1 is one of the most fluorescent NIR dyes. It has maximum absorption around 650 nm with extinction coefficient larger than 100,000 M-1 cm-1. It has fluorescence emission around 670 nm, which is pH-independent. Although Oxazine 1 is quite stable from pH 4 to pH 10, it might be reduced by some biological...
Application: Labeling
CAS 24796-94-9
114.00€ *
Review
BAPTA
BAPTA

Item number: Cay11706-100

BAPTA is a membrane-impermeable calcium chelator that binds extracellular calcium ions. The presence of four carboxylic acid functional groups allows for the binding of two calcium ions. BAPTA and its derivatives are widely used for the measurement and manipulation of intracellular free calcium concentration.Formal...
Keywords: N,N'-[1,2-ethanediylbis(oxy-2,1-phenylene)]bis[N-(carboxymethyl)-glycine
Application: Calcium chelator
CAS 85233-19-8
MW: 476.4 D
From 51.00€ *
Review
Cal-520FF(TM), AM
Cal-520FF(TM), AM

Item number: ABD-21142

Ex: 492 nm. Em: 514 nm. Soluble in: DMSO. Cal-520T provides a robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization. Cal-520T AM is a new fluorogenic calcium-sensitive dye with a significantly improved signal to noise ratio and intracellular retention compared to the...
Application: Cell-based assays, calcium indicator
MW: 1138,92 D
From 507.00€ *
Review
XTT (sodium salt hydrate)
XTT (sodium salt hydrate)

Item number: Cay14919-250

XTT is a cell-impermeable, negatively charged tetrazolium dye that produces a water-soluble formazan when reduced at the cell surface by cellular-derived NADH and an electron mediator. It is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.Formal Name:...
Keywords: 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium, inner salt, monosodium salt, hydrate
Application: Cell proliferation studies
CAS 413585-64-5
MW: 650.5 D
From 87.00€ *
Review
HIS Lite(TM) Cy3 Bis NTA-Ni Complex
HIS Lite(TM) Cy3 Bis NTA-Ni Complex

Item number: ABD-12610

HIS Lite(TM) Cy3 Bis NTA-Ni Complex provides specific and highly sensitive detection of His-tagged fusion proteins. Ex 555 nm. Em: 565 nm.
Application: Fluorescent His-Tag detection
581.00€ *
Review
Cyanine 3 monosuccinimidyl ester [equivalent to Cy3(R) NHS ester]
Cyanine 3 monosuccinimidyl ester [equivalent to Cy3(R)...

Item number: ABD-141

Ex: 555 nm. Em: 565 nm. Soluble in: DMSO. A variety of cyanine 3 (Cy3®) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy3® dyes have enhanced fluorescence upon binding...
Application: Labeling probe, classic labeling dye, cyanine
MW: 829,03 D
114.00€ *
Review
Protonex(TM) Red 600-Latex Bead Conjugate
Protonex(TM) Red 600-Latex Bead Conjugate

Item number: ABD-21209

Ex: 575 nm. Em: 597 nm. ProtonexT Red-latex bead conjugate demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of ProtonexT Red-latex bead conjugate. The fluorescence of ProtonexT Red-latex bead...
Application: Cell-based assays, pH indicator
434.00€ *
Review
FDA (Fluorescein diacetate)
FDA (Fluorescein diacetate)

Item number: ABD-22020

Can be used as a cell permeable marker of cell viability. It can pass through the cell membrane whereupon intracellular esterases hydrolyze the diacetate group producing the highly fluorescent product fluorescein.
Application: Cell viability assays
CAS 596-09-8
114.00€ *
Review
FMOC-Lys(Cy5)-OH
FMOC-Lys(Cy5)-OH

Item number: ABD-5055

FMOC-Lys(Cy5)-OH is a building block for in-sequence Lys labeling by Cy5. Cy5 is one of the most common fluorescent dyes for preparing FRET peptides (often paired with Cy3 or TQ5).
Application: Fluorescent labeling
1,019.00€ *
Review
Resorufin, sodium salt *Fluorescence reference standard*
Resorufin, sodium salt *Fluorescence reference standard*

Item number: ABD-65

Resorufin is used as a calibration standard for resorufin-derived enzyme substrates. It has pH dependent absorption and emission over the range of 5 to 9. It is also the reduction product of resazurin in cells.
Application: Labeling
CAS 34994-50-8
114.00€ *
Review
Phalloidin-iFluor(TM) 350 Conjugate
Phalloidin-iFluor(TM) 350 Conjugate

Item number: ABD-23110

This blue fluorescent phalloidin conjugate (equivalent to Alexa Fluor(R) 350-labeled phalloidin) selectively binds to F-actins. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections,...
Application: Labeling
MW: 400,00 D
218.00€ *
Review
Fluorescein dibutyrate
Fluorescein dibutyrate

Item number: CDX-F0057-G005

Solid. Soluble in DMSO, DMF or chloroform. Fluorogenic substrate for esterase and lipase.
Application: Fluorogenic esterase / lipase substrate
CAS 7298-65-9
MW: 472,49 D
From 168.00€ *
Review
33 from 269 pages