Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an... read more »
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Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

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Hoechst 33342 *20 mM solution in water*
Hoechst 33342 *20 mM solution in water*

Item number: ABD-17535

Ex (nm): 350. Em (nm): 461. The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy.
Application: Nucleic acid staining
145.00€ *
Review
BCECF AM
BCECF AM

Item number: Cay15922-1

BCECF AM ester is a widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two...
Keywords: BCECF Acetoxymethyl ester, 3',6'-bis[(acetyloxy)methoxy]-5(or...
Application: Fluorescent intracellular pH indicator
MW: 880.7 D
From 239.00€ *
Review
Ethidium homodimer
Ethidium homodimer

Item number: CDX-E0012-M001

Dark red solid. Soluble in water, DMSO or DMF. Staining dye for ssDNA, dsDNA, RNA, oligonucleotides and triplex DNA. It does not cross intact cell membranes and can be used to test cell viability. Reagent for the fluorimetric detection of nucleic acids.
Keywords: EthD-1, EtDi
Application: Fluorimetric nucleic acid detection, ssDNA, dsDNA, RNA, oligonucleotides, triplex DNA staining dye
CAS 61926-22-5
MW: 856,75 D
From 246.00€ *
Review
Nuclear Red(TM) LCS1
Nuclear Red(TM) LCS1

Item number: ABD-17542

Our Nuclear Red(TM) LCS1 is a non-fluorescent, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. The Nuclear Red(TM) LCS1 has its orange fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This...
Application: DNA Labeling
MW: 650,00 D
145.00€ *
Review
Phalloidin-iFluor(TM) 633 Conjugate
Phalloidin-iFluor(TM) 633 Conjugate

Item number: ABD-23125

This deep red fluorescent phalloidin conjugate (equivalent to Alexa Fluor(R) 633-labeled phalloidin) selectively binds to F-actins. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue...
Application: Labeling
MW: 2,10 kD
218.00€ *
Review
DPPH
DPPH

Item number: Cay14805-50

2,2-Diphenyl-1-picrylhydrazyl (DPPH) is a stable free radical and colorimetric probe for the detection of free radical scavengers. Upon reaction with antioxidants, DPPH turns from deep violet to yellow, which can be quantified by colorimetric detection at 515 nm as a measure of antioxidant capacity. DPPH has...
Keywords: 2,2-Diphenyl-1-Picrylhydrazyl, DPPH radical, 2,2-diphenyl-1-(2,4,6-trinitrophenyl)-hydrazinyl
Application: Colorimetric antioxidant assay
CAS 1898-66-4
MW: 394.3 D
From 70.00€ *
Review
CytoTell(TM) Green
CytoTell(TM) Green

Item number: ABD-22253

Ex: 511 nm. Em: 525 nm. Soluble in: DMSO. Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations. Among all the existing fluorescent dyes CFSE is the preferred cell proliferation indicator that is widely used for live cell analysis. However, there are a few...
Application: Cell-based assays, fluorescent cell labeling, FC
MW: ~500 D
From 145.00€ *
Review
MitoLite(TM) Blue FX490
MitoLite(TM) Blue FX490

Item number: ABD-22674

Ex: 350 nm. Em: 490 nm. MitoLite(TM) Blue is a cationic dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in mitochondria after it gets into cells....
Application: FC
145.00€ *
Review
Fluorescein-5-maleimide
Fluorescein-5-maleimide

Item number: ABD-130

Maleimides are among the most frequently used reagents for thiol modification. In most proteins, the site of reaction is at cysteine residues that either are intrinsically present or resulted from reduction of cystines.
Application: Labeling
CAS 75350-46-8
185.00€ *
Review
Gly-Pro-AMC
Gly-Pro-AMC

Item number: ABD-13450

Gly-Pro-AMC is a sensitive fluorogenic substrate for detecting dipeptidyl peptidase IV (DPPIV), a serine protease that cleaves N-terminal dipeptides from polypeptides with L-proline or L-alanine at the penultimate position.
Application: Protease assay
CAS 115035-46-6
145.00€ *
Review
Cal-590(TM)-Dextran Conjugate *MW 3,000*
Cal-590(TM)-Dextran Conjugate *MW 3,000*

Item number: ABD-20508

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and...
Application: IF, FC
MW: ~4000 D
434.00€ *
Review
Cal-630 (TM) potassium salt
Cal-630 (TM) potassium salt

Item number: ABD-20538

Ex: 608 nm. Em: 626 nm. Soluble in: water. Cal-630(TM) has been developed to improve x-Rhod-1 cell loading and calcium response while maintaining the spectral wavelength of x-Rhod-1, making it compatible with Texas Red(R) filter set. In CHO and HEK cells Cal-630(TM) AM has cellular calcium response that is much more...
MW: 1139,11 D
291.00€ *
Review
31 from 269 pages