Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an... read more »
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Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

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mFluor(TM) Green 615 SE
mFluor(TM) Green 615 SE

Item number: ABD-1166

Extinction coefficient (cm^-1 M^-1): 40000 (PBS). Excitation (nm): 530. Emission (nm): 612. mFluor(TM) Green 615 dye can be well excited with green laser at 532 nm. It has a huge Stokes shift with emission ~615 nm. mFluor(TM) Green 615 dyes are water-soluble, and the protein conjugates prepared with mFluor(TM) Green...
Application: Red fluorescent dye, green laser, FC
MW: 815.87 D
416.00€ *
Review
HIS Lite(TM) Cy3 Bis NTA Chelator
HIS Lite(TM) Cy3 Bis NTA Chelator

Item number: ABD-12650

Correction Factor (260 nm) 0.07. Correction Factor (280 nm) 0.073. Extinction coefficient (cm^-1 M^-1): 150000 (PBS). Excitation (nm): 555. Emission (nm): 569. Quantum yield 0.15 (PBS). Cy3 Bis NTA-Ni Chelator can be applied either to an SDS-PAGE gel or Western blot membrane for fluorescence imaging or used in...
Application: Fluorescent His-tag imaging
MW: 1177.3 D
581.00€ *
Review
HIS Lite(TM) Cy5 Bis NTA Chelator
HIS Lite(TM) Cy5 Bis NTA Chelator

Item number: ABD-12653

Correction Factor (260 nm) 0.02. Correction Factor (280 nm) 0.03. Correction Factor (482 nm) 0.009. Correction Factor (565 nm) 0.09. Extinction coefficient (cm^-1 M^-1): 250000 (ethanol). Excitation (nm): 651. Emission (nm): 670. Quantum yield 0.27 (PBS), 0.4 (ethanol). Cy5 Bis NTA-Ni Chelator can be applied either...
Application: Fluorescent His-tag imaging
MW: 1203.34 D
581.00€ *
Review
HIS Lite(TM) OG488-Tris NTA Chelator
HIS Lite(TM) OG488-Tris NTA Chelator

Item number: ABD-12655

Correction Factor (260 nm) 0.31. Correction Factor (280 nm) 0.12. Extinction coefficient (cm^-1 M^-1): 76000. Excitation (nm): 498. Emission (nm): 526. HIS Lite(TM) OG488-Tris NTA Chelator is used as a sensitive green fluorescent probe for detecting polyhistidine-labeled proteins in combination with the addition of...
Application: Fluorescent His-tag imaging
MW: 1443.34 D
581.00€ *
Review
JUN Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
JUN Dye qPCR Calibration Plate *Optimized for ABI7500...

Item number: ABD-67018

Extinction coefficient (cm^-1 M^-1): 110000 (aqueous buffer (pH 7.2)). Excitation (nm): 610. Emission (nm): 628. Quantum yield 0.85 (aqueous buffer (pH 7.2)). The JUN Dye qPCR Calibration Plate is designed for use with the 7500 Real-Time PCR system featuring a Fast 96-well block. It facilitates the calibration and...
Application: 7500 Real-Time PCR system fast 96-well block calibration
124.00€ *
Review
MP650 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
MP650 Dye qPCR Calibration Plate *Optimized for ABI7500...

Item number: ABD-67019

Extinction coefficient (cm^-1 M^-1): 250000 (aqueous buffer (pH 7.2)). Excitation (nm): 639. Emission (nm): 653. Quantum yield 0.29 (aqueous buffer (pH 7.2)). MP650 is designed to have identical spectra to those of Mustang Purple. The MP650 Dye qPCR Calibration Plate is designed for use with the 7500 Real-Time PCR...
Keywords: Mustang purple equivalent
Application: 7500 Real-Time PCR system fast 96-well block calibration
124.00€ *
Review
SYBR Dye qPCR Calibration Solution *10,000X*
SYBR Dye qPCR Calibration Solution *10,000X*

Item number: ABD-67035

Excitation (nm): 498. Emission (nm): 522. Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal. Since instruments can differ in fluorescence detection sensitivity, the concentrations needed to calibrate any dye, such as SYBR, will depend on the device used. The AAT...
Application: ABI7500 Fast, QuantStudio(TM), and ViiA(TM) 7 system Real-Time PCR system calibration
395.00€ *
Review
TAMRA Dye qPCR Calibration Solution *10,000X*
TAMRA Dye qPCR Calibration Solution *10,000X*

Item number: ABD-67036

Correction Factor (260 nm) 0.32. Correction Factor (280 nm) 0.178. Extinction coefficient (cm^-1 M^-1): 90000. Excitation (nm): 552. Emission (nm): 578. Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal. Since instruments can differ in fluorescence detection...
Application: ABI7500 Fast, QuantStudio(TM), and ViiA(TM) 7 system Real-Time PCR system calibration
395.00€ *
Review
JUN Dye qPCR Calibration Solution *10,000X*
JUN Dye qPCR Calibration Solution *10,000X*

Item number: ABD-67038

Extinction coefficient (cm^-1 M^-1): 110000 (aqueous buffer (pH 7.2)). Excitation (nm): 610. Emission (nm): 628. Quantum yield 0.85 (aqueous buffer (pH 7.2)). The JUN Dye qPCR Calibration Plate is designed for use with the 7500 Real-Time PCR system featuring a Fast 96-well block. It facilitates the calibration and...
Application: 7500 Real-Time PCR system fast 96-well block calibration
395.00€ *
Review
MP650 Dye qPCR Calibration Solution *10,000X*
MP650 Dye qPCR Calibration Solution *10,000X*

Item number: ABD-67039

Extinction coefficient (cm^-1 M^-1): 250000 (aqueous buffer (pH 7.2)). Excitation (nm): 639. Emission (nm): 653. Quantum yield 0.29 (aqueous buffer (pH 7.2)). MP650 is designed to have identical spectra to those of Mustang Purple. Real-time thermal cyclers require calibration to generate a spectral profile for the...
Keywords: Mustang purple equivalent
Application: ABI7500 Fast, QuantStudio(TM), and ViiA(TM) 7 system Real-Time PCR system calibration
395.00€ *
Review
OG514 acid [equivalent to Oregon Green(R) 514 carboxylic acid]
OG514 acid [equivalent to Oregon Green(R) 514 carboxylic...

Item number: ABD-716

Correction Factor (280 nm) 0.19. Extinction coefficient (cm^-1 M^-1): 85000. Excitation (nm): 513. Emission (nm): 533. OG514 is the same fluorophore as Oregon Green 514 (Oregon Green(R) is the trademark of ThermoFisher). OG514 acid is an amine-reactive compound used for creating green, fluorescent bioconjugates. It...
Keywords: Oregon Green 514 equivalent
Application: Green fluorescent label, amine-reactive
MW: 512.36 D
375.00€ *
Review
OG514 succinimidyl ester [equivalent to Oregon Green(R) 514 succinimidyl ester]
OG514 succinimidyl ester [equivalent to Oregon Green(R)...

Item number: ABD-717

Correction Factor (280 nm) 0.19. Extinction coefficient (cm^-1 M^-1): 85000. Excitation (nm): 513. Emission (nm): 533. OG514 is the same fluorophore as Oregon Green 514 (Oregon Green(R) is the trademark of ThermoFisher). OG514 Succinimidyl Ester is an amine-reactive compound used for creating green, fluorescent...
Keywords: Oregon Green 514 equivalent
Application: Green fluorescent label, amine-reactive
MW: 609.43 D
375.00€ *
Review
264 from 267 pages